Product Overview

AMPPD is a chemiluminescent substrate for alkaline phosphatase (ALP). In a suitable buffer, it is hydrolyzed under enzymatic catalysis to form AMP-D, which emits strong luminescent signals. The luminescence rate is correlated with the concentration of ALP. When ALP is conjugated to hybridization probes, this system can be used to quantify hybridized molecules.

Key Features

Sensitive Detection

Capable of detecting ALP concentrations below 10⁻¹⁹ mol/mL and target proteins at levels as low as 5 pg, supporting applications requiring high analytical sensitivity.

Stable Signal Output

Provides a stable luminescent plateau for up to 60 minutes, allowing flexible signal acquisition and compatibility with various detection workflows.

Broad Linear Range

Demonstrates a strong linear relationship between ALP concentrations ranging from 50 to 50,000 pg/mL and photon counts, supporting quantitative analysis across a wide dynamic range.

Good Storage Stability

Stable for up to 2 years when stored at 2–8°C protected from light, facilitating routine use and inventory management.

Compatible with ALP-Based Detection Systems

Designed for use in alkaline phosphatase-labeled assays, including applications involving ALP-conjugated probes and detection reagents.

Applications

ALP-Based Chemiluminescent Detection

Suitable for chemiluminescent assays utilizing alkaline phosphatase as the reporter enzyme, enabling sensitive signal generation.

Immunodiagnostic Assays

Applicable to a variety of immunodiagnostic testing formats based on ALP labeling.

Nucleic Acid Hybridization Assays

Can be used with ALP-conjugated hybridization probes to quantify hybridized nucleic acid targets.

Molecular Biology Research

Suitable for molecular biology applications requiring chemiluminescent detection, including enzyme activity studies and analytical assays.

Performance Data

Low Background and Ultra-high Signal-to-noise Ratio, Enhancing Detection Sensitivity

Comparison of Signal-to-Noise Ratio Measured Values of AMPPD Chemiluminescent Substrates from Different Manufacturers

Luminescence Kinetic Curves of AMPPD Chemiluminescent Substrates from Different Brands

Compared with peer products, Hzymes AMPPD chemiluminescent substrate solution delivers significantly lower background signals and an ultra-high signal-to-noise ratio, enabling improved detection sensitivity and more reliable assay performance.

Excellent Thermal Stability, Ensuring Long-term Reagent Stability

After 28-day accelerated test at 37 °C, the change in AMPPD chemiluminescence value was ≤10%.

Hzymes AMPPD chemiluminescent substrate solution demonstrates excellent thermal stability, maintaining consistent performance under different storage and application conditions, thereby enhancing reagent stability and extending usability.

High Detection Repeatability and Excellent Batch-to-batch Consistency

Batch-to-batch performance evaluation of Hzymes AMPPD chemiluminescent substrate solution showed a coefficient of variation (CV) of ≤5%, demonstrating outstanding detection repeatability, reliable assay consistency, and excellent product quality control.

Note: Test conditions: 5 μL enzymes + 150 μL substrate solution, enzyme activity 0.07 MU. All data were obtained using Lumistation 1800.

FAQ

What are the recommended storage conditions and shelf life of this product?

The product should be stored at 2–8°C in a sealed, dry environment and protected from light. Please refer to the product specification sheet for validated shelf-life information.

What is the recommended usage or dosage for this product?

The recommended concentration is 150–300 mg/L. The optimal dosage should be determined according to the customer's assay formulation and solution replacement strategy.

Is this product compatible with different ALP-based detection systems?

Yes. This substrate is suitable for a variety of alkaline phosphatase-based chemiluminescent assays, including immunodiagnostic applications and nucleic acid hybridization assays utilizing ALP-conjugated probes.

Do you provide OEM or private-label manufacturing services?

Yes. We offer OEM and private-label manufacturing services to support customers' formulation, packaging, and branding requirements.

What are the shipping conditions and typical lead times?

The product is typically shipped under temperature-controlled conditions with appropriate protection from light.

In stock: Typical lead time is 2–3 weeks

Out of stock: Typical lead time is 8–12 weeks