Product Overview

Cas9 mRNA encodes the Streptococcus pyogenes Cas9 (SpCas9) protein, one of the most widely used CRISPR-associated nucleases for genome editing applications. As the core component of the CRISPR-Cas9 system, Cas9 protein forms a complex with single guide RNA (sgRNA) to recognize target DNA sequences and induce precise double-strand DNA breaks (DSBs) at specific genomic loci.

This Cas9 mRNA encodes a SpCas9 protein containing nuclear localization signals (NLS) at both the N- and C-termini, facilitating efficient nuclear transport after translation and enhancing DNA cleavage activity. A C-terminal HA epitope tag is incorporated to enable convenient detection, identification, and purification of the expressed Cas9 protein.

The product is synthesized through in vitro co-transcriptional capping and purified by precipitation and desalting processes. With a theoretical length of 4522 nt, Cas9 mRNA enables efficient and sustained protein expression in mammalian cells, making it suitable for CRISPR-based genome editing research and functional studies.

Amino Acid Sequence of Encoded Protein：

MAPKKKRKVGIHGVPAADKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKNLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQIGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQLGGDSGGKRPAATKKAGQAKKKKGSYPYDVPDYA*

Key Features

High-Quality SpCas9 mRNA for Genome Editing

Encodes a functional Streptococcus pyogenes Cas9 nuclease optimized for CRISPR-Cas9-mediated DNA cleavage and genome editing applications.

Efficient Nuclear Localization and Protein Expression

Contains NLS sequences at both N- and C-termini to promote nuclear import of translated Cas9 protein and improve genome editing efficiency.

Convenient Protein Detection

Integrated C-terminal HA tag supports straightforward detection, analysis, and purification of expressed Cas9 protein.

Reliable Stability and Supply

• Stable for 1 month at 2–8°C

• Tolerates more than 10 freeze-thaw cycles

• Short lead time with customization services available

Applications

Cas9 mRNA is suitable for a broad range of CRISPR-Cas9 genome editing applications, including:

• Gene knockout and gene disruption studies

• Targeted genome editing in mammalian cells

• Functional genomics research

• Disease model development

• Gene regulation and validation studies

• CRISPR-based cellular assays

• In vivo animal genome editing studies

Performance Data

Western Blot Analysis of Cas9 Protein Expression in 293T Cells

Stability of mRNA Bulk Solution at 2–8°C

Cas9 mRNA maintains stability during refrigerated storage, supporting flexible laboratory handling and short-term storage.

Freeze-Thaw Stability

Cas9 mRNA retains integrity and performance after repeated freeze-thaw cycles, demonstrating excellent formulation stability.

Long-Term Stability at -80°C

The product maintains stability during long-term frozen storage at -80°C, ensuring reliable performance for downstream applications.

FAQ

Q1: What protein does Cas9 mRNA encode?

Cas9 mRNA encodes the Streptococcus pyogenes Cas9 (SpCas9) protein, a widely used nuclease in CRISPR-Cas9 genome editing systems.

Q2: Does this Cas9 mRNA contain nuclear localization signals (NLS)?

Yes. The encoded Cas9 protein contains NLS sequences at both N- and C-termini to facilitate nuclear transport and enhance DNA cleavage efficiency.

Q3: What is the storage condition for Cas9 mRNA?

Cas9 mRNA is recommended to be stored at -85°C to -65°C. Transportation is supported under -45°C to 25°C conditions.

Q4: Can Cas9 mRNA be customized?

Yes. Custom sequence design and formulation services are available upon request.

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