| Cat. No | Size |
| HMD9901-01 | 192T |
| HMD9901-02 | 48T |
Expected Usage
This kit is used to detect DNase contamination in samples.
Attention
1. The sample adding operation should be as fast
as possible. Too long time will affect the
accuracy of the experiment.
2. The parameters of different fluorescent enzyme labeling instruments are different. Set appropriate gain before the first test.
3. All reagents shall be fully shaken before use. When adding samples, the added samples shall
be added to the bottom of the enzyme label plate well to avoid adding them to the upper part of
the well wall. When adding samples, pay
attention not to splash or generate bubbles.
4. The standard in the kit is DNase I, and its active unit is defined as that one unit is defined
as the amount of enzyme which will completely
degrade 1 µg of pBR322 DNA in 10 minutes at
37°C in DNase I Reaction Buffer[1]. One DNase
I unit is equivalent to 0.3 Kunitz unit[2]. 。
5. In order to avoid exogenous DNase
contamination, DNase RNase away can be
sprayed on the surface of the experimental table, gloves and other surfaces. After 5 minutes, clean
them with clean paper towels and then carry out subsequent experimental operations.
