Specification
| Appearance | Yellow amorphous powder, lyophilized |
| Protein purity | ≥90% (from SDS-PAGE) |
| Activity | ≥4 U/mg solid |
| Catalase | ≤0.001% |
| ATPase | ≤0.005% |
| Glucose oxidase | ≤0.03% |
| Cholesterol oxidase | ≤0.003% |
Properties
| EC number | 1.5.3 (Recombinant from microorganism) | |
| Molecular weight | 55 kDa (SDS-PAGE) | |
| Isoelectric point | 6 | |
| Michaelis constants | 5.0×10-4M（fructosyl-Ala） | |
| Inhibitors | Hg2+,Pb2+ | |
| Optimum pH | 7.7 | Fig.1 |
| Optimum temperature | 42℃ | Fig.2 |
| pHstability | 5.0-9.5(25℃,16h) | Fig.3 |
| Thermal stability | Below40℃(pH8.0,30min) | Fig.4 |
| Storage stability | At least one year at -25～-15℃ | Fig.5 |
| Stabilizers | Glycerol,Trehalose |
Applications
The enzyme is useful for the determination of fructosyl-L-amino acid.
Unit definition
One unit (U) is defined as the amount of enzyme which produces 1 μmol of H2O2 per min under the conditions described below.
