Product Overview

Hzymes recombinant enterokinase (rEK) is expressed in E. coli and contains a His-tag. It has been mutated based on the native enterokinase light chain to achieve higher specific activity and cleavage specificity, making it especially suitable for production of peptide drugs such as GLP-1 and its analogs.

Key Features

High Cleavage Specificity

Engineered based on the native enterokinase light chain to provide enhanced specificity toward the DDDDK recognition sequence, helping reduce non-specific cleavage.

Efficient DDDDK Sequence Cleavage

Specifically recognizes and cleaves DDDDK-tagged proteins and peptides, supporting reliable tag removal during downstream processing.

Recombinant E. coli Expression

Produced using a recombinant E. coli expression system and supplied with a His-tag to support manufacturing consistency and quality control.

Suitable for Peptide Processing

Designed for enzymatic cleavage applications requiring high specificity and reproducible performance in peptide production workflows.

Applications

GLP-1 and Peptide Drug Preparation

Suitable for the production and preparation of peptide therapeutics, including GLP-1 and related peptide analogs.

Fusion Tag Removal

Applicable to the specific removal of DDDDK fusion tags from recombinant proteins and peptides during purification and processing.

Peptide Process Development

Supports process development and optimization involving enterokinase-mediated cleavage steps.

Recombinant Protein Manufacturing

Can be integrated into recombinant protein production workflows where site-specific proteolytic cleavage is required.

Performance Data