Product Overview

Co-capping T7 in vitro transcription reagent is a co-transcription capping reagent for in vitro transcription of 5'capped mRNA to produce Cap-1 structure with an optimized RNA synthesis formulation.

The kit is capable of synthesizing mRNAs that contain a natural Cap-1 structure in a single simplified reaction without compromising RNA yield using linear double-stranded DNA containing T7 promoter followed by an AG initiation sequence.

The kit contains modified nucleotides pUTP as alternative. Capped mRNAs with modified nucleotide have lower immunogenic potential.

Each standard reaction yields up to 150-200 μg of RNA from 1μg DNA template. Reaction size can be scaled up to yield milligram-level RNA as needed.

RNA synthesized from the kit is suitable for many applications including RNA structure and function studies, transfections, microinjections, in vitro translation, preclinical mRNA therapeutic mRNA studies.

Key Features

High Yield

Generates up to 180-220 μg of capped mRNA from 1 μg of DNA template under standard reaction conditions, supporting efficient RNA production and scalable applications.

One-Step Cap-1 mRNA Synthesis

Enables the generation of natural Cap-1 mRNA in a single co-transcriptional reaction using linear double-stranded DNA templates containing a T7 promoter and AG initiation sequence, without the need for additional capping procedures.

Compatible with pUTP Incorporation

Supports the incorporation of pUTP during in vitro transcription, enabling the preparation of modified capped mRNA transcripts.

Broad Template Compatibility

Supports DNA templates ranging from 200 bp to 12 kb, allowing the synthesis of RNA transcripts with varying lengths and sequence complexities.

Complete, Ready-to-Use Workflow

Provided as a pre-optimized IVT system containing all essential reagents, reducing hands-on preparation and simplifying experimental setup.

Applications

Modified Cap-1 mRNA Production

Suitable for the synthesis of Cap-1 mRNA incorporating pUTP through a streamlined co-transcriptional capping workflow.

mRNA Research and Preclinical Studies

Applicable to a range of applications, including preclinical mRNA studies, RNA functional studies, and IVT process development.

In Vitro Translation and Cellular Applications

The resulting capped mRNA transcripts can be used in in vitro translation, transfection, and microinjection experiments requiring high-quality RNA.

Simplified IVT Workflows

As a complete and pre-optimized transcription system, the kit minimizes manual reagent preparation and reduces the need for reaction optimization.

Performance Data

Yield Comparison & Integrity Comparison

dsRNA Content Comparison

Performance on Different Length Templates

Component List

FAQ

Can this kit generate Cap-1 mRNA in a single reaction?

Yes. The kit enables the synthesis of natural Cap-1 mRNA through a one-step co-transcriptional process, eliminating the need for separate post-transcriptional capping procedures.

Is this kit compatible with pUTP incorporation?

Yes. The kit supports the incorporation of pUTP during in vitro transcription, enabling the preparation of modified Cap-1 mRNA transcripts.

Does this kit require a specific template design?

Yes. For optimal performance, the kit is designed for use with linear double-stranded DNA templates containing a T7 promoter followed by an AG initiation sequence.

What applications is this kit suitable for?

The kit is suitable for preclinical mRNA studies, RNA functional studies, in vitro translation, transfection, microinjection, and other applications requiring Cap-1 mRNA.

How should the kit be stored and transported?

The kit should be transported below 0°C and stored at -25 to -15°C to maintain optimal stability and performance.