Product Overview

Hi-yield T7 in vitro transcription reagent kit (Thermostable) is capable of in vitro transcription at elevated temperatures up to 50°C.

The kit is capable of synthesizing high yield RNA transcripts using linear double-stranded DNA containing T7 promoter as template and NTPs as substrate.

The kit is suitable for incorporation of modified nucleotides to obtain biotin labeled, dye labeled and radiolabeled RNA. The kit also can be applied in synthesis of co-transcriptionally capping mRNAs with cap analogs. The kit contains modified nucleotides N1-Me-pUTP as alternative.

Each standard reaction yields up to 150-200 μg of RNA from 1 μg DNA template. Reaction size can be scaled up to yield milligram-level RNA as needed.

RNA synthesized from the kit is suitable for many applications including RNA structure and function studies, ribozyme biochemistry, RNase protection assays and hybridization-based blots, anti-sense RNA and RNAi experiments.

RNA synthesized from the kit also is suitable for enzymatic production of capped RNA by vaccinia capping enzyme (HBP000606) and 2’-O-Methyltransferase (HBP000701) and being polyadenylated with Poly(A) polymerase (HBP000801) to improve the stability and translational competence of RNA used for in vitro translation, transfection, and microinjection.

Key Features

High-Yield RNA Synthesis

Generates up to 180-220 μg of RNA from 1 μg of DNA template under standard reaction conditions, supporting scalable RNA production from research to manufacturing applications.

Broad Temperature Compatibility

Powered by an engineered thermostable T7 RNA Polymerase, the kit maintains robust transcription performance across a wide temperature range of 37°C to 55°C, enabling seamless integration into both conventional and high-temperature IVT workflows.

Enhanced Performance with Challenging Templates

Elevated-temperature transcription helps minimize the impact of template secondary structures, improving transcription efficiency for GC-rich, structurally complex, and long RNA templates.

Broad Compatibility with IVT Modifications

Compatible with a wide range of modified nucleotides and cap analogs, including N1-Me-pUTP, enabling the synthesis of diverse RNA modalities for research and therapeutic applications.

Complete, Ready-to-Use IVT System

Supplied as a fully optimized transcription system containing all essential reagents, eliminating manual formulation and reducing workflow setup time.

Applications

High-Temperature IVT Process Development

Designed specifically for elevated-temperature in vitro transcription, the kit expands process development flexibility and enables optimization beyond conventional 37°C conditions.

Complex Template Transcription

The kit utilizes an engineered thermostable T7 RNA Polymerase mutant (Cat. No. HBP000350) to efficiently transcribe templates containing stable secondary structures, high GC content, or other challenging sequence features.

circRNA Production Workflows

Particularly suitable for circular RNA (circRNA) applications, supporting the generation of high-quality precursor transcripts that may improve downstream self-circularization efficiency.

GMP Manufacturing and Preclinical Development

Suitable for the development and production of mRNA vaccines, gene therapies, circRNA therapeutics, and other RNA-based products in both preclinical and GMP manufacturing settings.

Performance Data

Maintains high transcriptional activity at elevated temperatures

Delivers superior compatibility with circular RNA synthesis workflows

Component List

FAQ

Why choose the Thermostable IVT Kit over conventional IVT systems?

This kit supports in vitro transcription at temperatures up to 50°C, helping overcome template secondary structures and expanding IVT process flexibility while maintaining high RNA yield.

What applications is this kit suitable for?

The kit is suitable for mRNA vaccine development, gene therapy research, circRNA production, and other RNA applications requiring high-temperature IVT workflows or challenging templates.

Can this kit be used with modified nucleotides and cap analogs?

Yes. The kit is compatible with a broad range of modified nucleotides, including N1-Me-pUTP, as well as cap analogs for co-transcriptional capping.

Is this kit suitable for templates with complex secondary structures?

Yes. Elevated-temperature transcription can reduce the impact of stable secondary structures, enabling more efficient transcription of GC-rich, structurally complex, and long templates.

How should the kit be stored and transported?

The kit should be transported below 0°C and stored at -25 to -15°C to maintain optimal stability and performance.