Product Overview

Co-capping T7 in vitro transcription reagent is a co-transcription capping reagent for in vitro transcription of 5'capped mRNA to produce Cap-1 structure with an optimized RNA synthesis formulation.

The kit is capable of synthesizing mRNAs that contain a natural Cap-1 structure in a single simplified reaction without compromising RNA yield using linear double-stranded DNA containing T7 promoter followed by an AG initiation sequence.

The kit contains modified nucleotides N1-Me-pUTP as alternative. Capped mRNAs with modified nucleotide have lower immunogenic potential.

Each standard reaction yields up to 150-200 μg of RNA from 1μg DNA template. Reaction size can be scaled up to yield milligram-level RNA as needed.

RNA synthesized from the kit is suitable for many applications including RNA structure and function studies, transfections, microinjections, in vitro translation, preclinical mRNA therapeutic mRNA studies.

Key Features

High Yield

Generates up to 180-220 μg of capped mRNA from 1 μg of DNA template under standard reaction conditions, supporting efficient RNA production and scalable applications.

One-Step Cap-1 mRNA Synthesis

Enables the synthesis of natural Cap-1 mRNA in a single co-transcriptional reaction using linear double-stranded DNA templates containing a T7 promoter and AG initiation sequence, without additional post-transcriptional capping steps.

Compatible with N1-Me-pUTP Incorporation

Supports the incorporation of N1-Me-pUTP during IVT, providing flexibility for the preparation of modified mRNA transcripts.

Broad Template Compatibility

Supports DNA templates ranging from 200 bp to 12 kb, enabling the generation of RNA transcripts of varying lengths.

Applications

Modified Cap-1 mRNA Production

Suitable for the synthesis of Cap-1 mRNA incorporating N1-Me-pUTP in a streamlined co-transcriptional workflow.

mRNA Research and Preclinical Studies

Applicable to a variety of mRNA-related applications, including preclinical mRNA therapeutic studies, RNA functional studies, and process development.

In Vitro Translation and Cellular Applications

The resulting capped mRNAs can be used in in vitro translation, transfection, and microinjection experiments requiring high-quality RNA transcripts.

Simplified IVT Workflows

Supplied as a complete, pre-optimized IVT system containing all required reagents, reducing manual preparation and minimizing reaction optimization efforts.

Performance Data

Yield Comparison & Integrity Comparison

dsRNA Content Comparison

Performance on Different Length Templates

Component List

FAQ

Can this kit generate Cap-1 mRNA in a single reaction?

Yes. The kit enables the synthesis of natural Cap-1 mRNA through a one-step co-transcriptional process, eliminating the need for separate post-transcriptional capping procedures.

Is this kit compatible with N1-Me-pUTP incorporation?

Yes. The kit supports the incorporation of N1-Me-pUTP during IVT for the preparation of modified Cap-1 mRNA transcripts.

Does this kit require a specific template design?

Yes. Optimal performance is achieved using linear double-stranded DNA templates containing a T7 promoter followed by an AG initiation sequence.

What applications is this kit suitable for?

The kit is suitable for preclinical mRNA studies, RNA functional studies, in vitro translation, transfection, microinjection, and other applications requiring Cap-1 mRNA.

How should the kit be stored and transported?

The kit should be transported below 0°C and stored at -25 to -15°C to maintain optimal stability and performance.