Product Overview
Hi-yield T7 in vitro transcription reagent kit is designed for in vitro transcription of RNA using T7 RNA polymerase. The kit is capable of synthesizing high yield RNA transcripts using linear double-stranded DNA containing T7 promoter as template and NTPs as substrate.
The kit is suitable for incorporation of modified nucleotides to obtain biotin labeled, dye labeled and radiolabeled RNA. The kit also can be applied in synthesis of co-transcriptionally capping mRNAs with cap analogs. The kit contains modified nucleotides pUTP as alternative.
Each standard reaction yields up to 150-200 μg of RNA from 1μg DNA template. Reaction size can be scaled up to yield milligram-level RNA as needed.
RNA synthesized from the kit is suitable for many applications including RNA structure and function studies, ribozyme biochemistry, RNase protection assays and hybridization-based blots, anti-sense RNA and RNAi experiment.
RNA synthesized from the kit also is suitable for enzymatic production of capped RNA by vaccinia capping enzyme (HBP000606) and 2’-O-Methyltransferase (HBP000701), and for polyadenylation with Poly(A) polymerase (HBP000801) to improve the stability and translational competence of RNA used for in vitro translation, transfection, and microinjection.
Key Features
High-Yield RNA Synthesis
Generates up to 180-220 μg of RNA from 1 μg of DNA template under standard reaction conditions, enabling efficient RNA production and scalable manufacturing.
Superior Transcript Integrity
Powered by an engineered high-specific-activity T7 RNA Polymerase, the kit minimizes transcriptional side reactions and delivers RNA transcripts with excellent integrity and reproducibility.
Optimized for pUTP Incorporation
Compatible with pUTP and a broad range of modified nucleotides, enabling the synthesis of modified RNAs for diverse research and therapeutic applications.
Broad Compatibility
Supports the use of various cap analogs and co-transcriptional capping strategies, providing flexibility for different IVT workflows.
Flexible Template Support
Efficiently transcribes templates ranging from 200 bp to 12 kb, accommodating RNA products of varying lengths and complexities.
Complete, Ready-to-Use IVT Workflows
Supplied as a pre-optimized kit containing all essential reagents, eliminating manual formulation and reducing workflow setup time.
Applications
Modified RNA Production
Supports the incorporation of pUTP during IVT, enabling the generation of modified RNA transcripts for a variety of downstream applications.
mRNA Vaccine and Therapeutic Development
Suitable for the production of high-quality RNA for mRNA vaccine research, gene therapy development, and other RNA-based therapeutics.
High-Quality IVT Workflows
The kit incorporates an engineered high-specific-activity T7 RNA Polymerase mutant (Cat. No. HBP000330) to improve transcription efficiency, reduce undesired side reactions, and enhance both RNA yield and transcript integrity.
Streamlined Workflow Development
As a complete, pre-optimized transcription system, the kit minimizes reaction setup and optimization efforts, enabling users to rapidly obtain reproducible IVT products with high efficiency.
Performance Data
Yield Comparison & Integrity Comparison
dsRNA Content Comparison
Performance on Different Length Templates
Component List
| Component | Concentration | Volume |
| ATP | 100 mM | 100 μL |
| CTP | 100 mM | 100 μL |
| GTP | 100 mM | 100 μL |
| UTP | 100 mM | 100 μL |
| pUTP | 100 mM | 100 μL |
| 10 × Hi-Yield IVT Buffer A | / | 100 μL |
| 10 × Hi-Yield IVT Buffer B | / | 100 μL |
| Enzyme Mix | / | 100 μL |
| Control template 1 | 0.5 μg/μL | 10 μL |
| LiCl | 5 M | 1.4 mL |
| DNase I | 5 U/μL | 100 μL |
FAQ
Is this kit compatible with pUTP incorporation?
Yes. The kit supports the incorporation of pUTP and other modified nucleotides during in vitro transcription, enabling the synthesis of modified RNA for a variety of downstream applications.
What RNA yield can be achieved with this kit?
Under standard reaction conditions, the kit can generate up to 150-200 μg of RNA from 1 μg of DNA template. The reaction can also be scaled up to produce milligram quantities of RNA.
Please update the value if the final specification is 180-220 μg.
Is the kit compatible with cap analogs?
Yes. The kit supports co-transcriptional capping using cap analogs, allowing the generation of capped RNA transcripts.
What applications is this kit suitable for?
The kit is suitable for modified RNA production, mRNA vaccine research, gene therapy development, RNA labeling studies, and other applications requiring high-yield IVT products.
How should the kit be stored and transported?
The kit should be transported below 0°C and stored at -25 to -15°C to maintain optimal stability and performance.
