Introduction

Hzymes Biotech has upgraded its host residual DNA detection products, covering multiple cell types such as  CHO, E. coli, Vero, HEK293, Pichia pastoris, and mycoplasma. The primer and probe sequences of the kit are from the pharmacopoeia, so you don’t need to do a full set of validation experiments, just need to confirm the method. Developed based on a quantitative PCR method,   LODs can reach 0. 003 pg/μL with no cross-reactivity with other DNA, reducing false positives in the assay reaction. Strict production management system,   batch-to-batch difference controllable CV< 10%. Provide a complete set of solutions for manual extraction and automatic instrument extraction< /b40> , which can quickly complete the detection within 2 hours and ensure the accuracy of quantitative detection.

Performance

The primer probe sequences for HCDs of CHO, Vero, E. coli, and yeast are from pharmacopoeia

Simple and convenient

The finished product of the extraction reagent is assembled and used directly,   without the need to prepare reagents separately, and the whole set is stored stably at room temperature.   Fully automated instrument extraction with  pre-packaged kits  for flexible on-demand access.

Intended Use

The Vero DNA Assay Kit, which is produced by Hzymes, can be used as quantitative determination of Vero cellular residual DNA in biological products (e. g. recombinant protein, antibodies, vaccines, etc) with qPCR detection principle on TaqMan fluorescent probes. The kit has the characteristics of high-speed and high specificity.
The detection method can be traced back to China pharmacopoeia method.

Storage and Stability

1. Period of validity: 24 months under specified storage conditions.
2. Transportation and storage: Store at -25~ -15℃ for long term storage and use dry ice or ice bag to keep it cold during.