Product Overview
Proteinase K (Lyophilized Powder)
Proteinase K is a highly active recombinant serine protease derived from Tritirachium album, widely used in nucleic acid extraction, molecular diagnostics, and biopharmaceutical sample preparation. It exhibits broad substrate specificity and efficiently digests proteins under both native and denaturing conditions, including in the presence of detergents such as SDS and urea.
Developed through directed evolution technology, Hzymes Proteinase K delivers enhanced enzymatic activity, exceptional stability, and excellent compatibility with nucleic acid purification workflows. The product is free of detectable DNase, RNase, and nickase contamination, ensuring reliable performance in sensitive DNA and RNA applications.
With an annual production capacity of 1,500 kg, Hzymes is one of the largest domestic suppliers of recombinant Proteinase K in China, providing consistent quality and scalable supply for diagnostic and industrial applications.
Specification
| Appearance | White to off-white amorphous powder, lyophilized |
|---|---|
| Activity | ≥30 U/mg |
| DNase | None detected |
| RNase | None detected |
| Nickase | None detected |
Properties
| Properties | ||
|---|---|---|
| EC number | 3. 4. 21. 64 (Recombinantfrom Tritirachiumalbum) | |
| Molecular weight | 29kDa (SDS-PAGE) | |
| Isoelectric point | 7. 81 | |
| Optimum pH | 7. 0-12. 0 | Fig. 1 |
| Optimum temperature | 65℃ | Fig. 2 |
| pH stability | pH 4. 5-12. 5 (25℃, 16h) | Fig. 3 |
| Thermal stability | Below50℃ (pH8. 0, 30min) | Fig. 4 |
| Storage stability | Over 90% activity stored at 25℃ for 12months | Fig. 5 |
| Activators | SDS, urea | |
| Inhibitors | Diisopropylfluorophosphate; phenylmethylsulfonylfluoride | |
| Storage condition | Dry powder condition ≤8℃ valid for 36 months | |
Key Features
High Enzyme Activity
Direct enzyme evolution enables higher specific activity
High Salt Tolerance
Tolerates guanidine hydrochloride Guanidine isothiocyanate, and others
Residue-Free
Free from DNase, RNase and Nickase contamination
No Background
Microorganisms mNGS-grade version is bioburden-free
Applications
DNA and RNA Extraction
Efficiently degrades proteins and nucleases during nucleic acid purification, improving DNA and RNA recovery.
Molecular Diagnostic Reagents
Suitable for PCR, qPCR, RT-PCR, and other molecular diagnostic workflows requiring high-purity nucleic acid preparation.
Genetic Diagnostic Kits
Widely used in sample pretreatment and nucleic acid extraction processes for genetic testing applications.
Biopharmaceutical Processing
Removes protein impurities during the preparation and purification of biological products, including nucleic acid-based therapeutics.
Tissue and Cell Sample Preparation
Facilitates the extraction of non-protein components from biological samples through efficient protein digestion.
Performance Data
3-Year Room Temperature Stability
Excellent Guanidine Salt Tolerance
DNase, RNase and Nickase Free Purity
FAQ
What are the main characteristics of the lyophilized powder form?
It is DNase-free and designed for stable performance in nucleic acid sample processing applications.
What are the formulation options for Proteinase K?
Proteinase K is available in both liquid and lyophilized powder formulations, providing flexibility depending on application needs.
What is the storage advantage of lyophilized Proteinase K?
Lyophilized formulation supports improved stability and handling flexibility compared with liquid formulations, depending on storage conditions and use cases.
