Product Overview
Proteinase K (Lyophilized Powder for NGS) is a highly active serine protease designed for efficient protein degradation in nucleic acid extraction and molecular diagnostic workflows. It exhibits broad substrate specificity, preferentially hydrolyzing ester bonds and peptide bonds adjacent to the C-terminal of hydrophobic, sulfur-containing, and aromatic amino acids, enabling effective digestion of complex protein matrices into short peptides.
Structurally, Proteinase K contains the classical Asp39–His69–Ser224 catalytic triad characteristic of serine proteases. Its catalytic core is further stabilized by two Ca²⁺ binding sites, contributing to enhanced structural stability and sustained enzymatic activity under diverse reaction conditions.
This product is optimized for next-generation sequencing (NGS) sample preparation and high-purity nucleic acid extraction, supporting robust performance in demanding molecular biology and diagnostic workflows.
Key Features
High Enzyme Activity
Engineered through directed enzyme evolution to achieve higher specific activity and improved protein digestion efficiency.
High Salt Tolerance
Maintains strong enzymatic performance in chaotropic salt conditions, including guanidine hydrochloride and guanidine isothiocyanate.
Residue-Free Purity
Free from DNase, RNase, and Nickase contamination, ensuring integrity of nucleic acids during extraction and downstream applications.
No Background Contamination
mNGS-grade formulation with controlled bioburden for reliable performance in sensitive molecular workflows.
Lyophilized Stability
Lyophilized powder format supports improved handling, long-term stability, and room temperature storage suitability.
Applications
DNA and RNA Extraction
Efficient removal of proteins and nucleases during nucleic acid purification, improving DNA/RNA yield and quality.
Molecular Diagnostic Reagents
Suitable for PCR, qPCR, RT-PCR, and other molecular diagnostic workflows requiring high-purity nucleic acid templates.
Genetic Diagnostic Kits
Widely applied in sample pretreatment and nucleic acid extraction steps in genetic testing workflows.
Biopharmaceutical Processing
Used for removal of protein impurities during preparation and purification of nucleic acid-based therapeutics and biologics.
Tissue and Cell Sample Preparation
Facilitates efficient protein digestion in complex biological samples, enabling downstream molecular analysis.
Performance Data
3-Year Room Temperature Stability
Both lyophilized powder and solution forms demonstrate stable activity after storage at room temperature for up to three years, supporting robust long-term usability.
Excellent Guanidine Salt Tolerance
Maintains enzymatic activity in the presence of strong chaotropic agents, including guanidine hydrochloride and guanidine isothiocyanate, ensuring compatibility with lysis buffers.
High Purity (DNase / RNase / Nickase-Free)
Validated to be free of DNase, RNase, and Nickase contamination, ensuring nucleic acid integrity and minimizing downstream assay interference.
FAQ
What are the advantages of the lyophilized NGS version?
It is free of DNase contamination and designed for stability and usability in nucleic acid preparation workflows related to NGS applications.
Can Proteinase K be stored as a lyophilized powder?
Yes, Proteinase K is available in lyophilized powder form as part of the product line, providing flexibility in formulation and storage format.
What applications is this version intended for?
It is intended for nucleic acid sample processing workflows used in NGS-related applications.
