Introduction: RNA Research Demands Uncompromising Protection

In RNA-based research and manufacturing workflows such as mRNA in vitro transcription, RT-qPCR, and RNA sequencing, even trace levels of RNase contamination can compromise experimental success. Among all RNases, RNase A is particularly abundant and notoriously resistant to standard sterilization methods like autoclaving.

To ensure the stability and integrity of RNA throughout these sensitive processes, Hzymes Biotech offers a GMP-grade Murine RNase Inhibitor—a high-performance, non-animal-origin solution designed for consistent RNase inhibition under stringent conditions.

Why RNase Inhibition Is Critical in RNA Applications

RNases are omnipresent—on lab benches, in pipette tips, tubes, and even on human skin. Their persistence and stability, especially RNase A, pose a significant threat to RNA integrity. In applications such as cDNA synthesis, mRNA production, and polyribosome isolation, uncontrolled RNase activity can lead to RNA degradation and experimental failure.

Preventing this contamination is essential for accurate, reproducible, and scalable RNA workflows. That’s where recombinant murine RNase inhibitors come into play.

Hzymes' Murine RNase Inhibitor: Superior Stability and Performance

Hzymes’ RNase inhibitor is a recombinant murine protein engineered to form a 1:1 complex with RNase A/B/C, effectively neutralizing their activity. Compared to human or porcine RNase inhibitors, murine variants offer enhanced oxidation resistance, making them suitable for applications requiring low DTT concentrations, such as real-time PCR and in vitro transcription reactions.

Key Features:

• High Stability
Remains active after 10 freeze-thaw cycles
Maintains function after incubation at 55°C for 5 minutes

• High Potency with Less Enzyme
Strong RNase inhibition with minimal enzyme quantity
Efficient even in complex reaction systems

• Strict Quality Control Standards

  ○ Low residual HCP, HCD, metal ions, and endotoxins

  ○ Nuclease-free, non-animal origin (AOF) certified

  ○ Manufactured under GMP conditions

• Regulatory Support

  ○ DMF filing number: MF036854

  ○ Suitable for use in RNA therapeutics and IVD products

Applications of Hzymes RNase Inhibitor

• mRNA synthesis and vaccine development

• Real-time RT-PCR and qPCR

• cDNA synthesis and RNA isolation

• Polyribosome and ribosome profiling protocols

• Clinical-grade RNA-based product manufacturing

Whether you are developing RNA vaccines or working on gene expression profiling, Hzymes’ murine RNase inhibitor ensures consistent protection against RNase contamination—even under stressful lab conditions.

Performance Validated Under Real Conditions

Our in-house testing demonstrates that Hzymes' RNase inhibitor remains stable after repeated freeze-thaw cycles and short-term thermal stress, delivering reliable RNase suppression throughout.

This product meets the rigorous expectations of both academic researchers and industrial-scale RNA product developers, helping preserve sample quality and maximize experimental reproducibility.

Thermal Stability and Freeze-thaw Stability Test of Hzymes' RNase inhibitor (Murine) The enzyme activity is not affected after freeze-thaw for 10 times or thermostable when incubate at 55°C for 5 mins.

Introduction: RNA Research Demands Uncompromising Protection

In RNA-based research and manufacturing workflows such as mRNA in vitro transcription, RT-qPCR, and RNA sequencing, even trace levels of RNase contamination can compromise experimental success. Among all RNases, RNase A is particularly abundant and notoriously resistant to standard sterilization methods like autoclaving.

To ensure the stability and integrity of RNA throughout these sensitive processes, Hzymes Biotech offers a GMP-grade Murine RNase Inhibitor—a high-performance, non-animal-origin solution designed for consistent RNase inhibition under stringent conditions.

Why RNase Inhibition Is Critical in RNA Applications

RNases are omnipresent—on lab benches, in pipette tips, tubes, and even on human skin. Their persistence and stability, especially RNase A, pose a significant threat to RNA integrity. In applications such as cDNA synthesis, mRNA production, and polyribosome isolation, uncontrolled RNase activity can lead to RNA degradation and experimental failure.

Preventing this contamination is essential for accurate, reproducible, and scalable RNA workflows. That’s where recombinant murine RNase inhibitors come into play.

Hzymes' Murine RNase Inhibitor: Superior Stability and Performance

Hzymes’ RNase inhibitor is a recombinant murine protein engineered to form a 1:1 complex with RNase A/B/C, effectively neutralizing their activity. Compared to human or porcine RNase inhibitors, murine variants offer enhanced oxidation resistance, making them suitable for applications requiring low DTT concentrations, such as real-time PCR and in vitro transcription reactions.

Key Features:

• High Stability
Remains active after 10 freeze-thaw cycles
Maintains function after incubation at 55°C for 5 minutes

• High Potency with Less Enzyme
Strong RNase inhibition with minimal enzyme quantity
Efficient even in complex reaction systems

• Strict Quality Control Standards

  ○ Low residual HCP, HCD, metal ions, and endotoxins

  ○ Nuclease-free, non-animal origin (AOF) certified

  ○ Manufactured under GMP conditions

• Regulatory Support

  ○ DMF filing number: MF036854

  ○ Suitable for use in RNA therapeutics and IVD products

Applications of Hzymes RNase Inhibitor

• mRNA synthesis and vaccine development

• Real-time RT-PCR and qPCR

• cDNA synthesis and RNA isolation

• Polyribosome and ribosome profiling protocols

• Clinical-grade RNA-based product manufacturing

Whether you are developing RNA vaccines or working on gene expression profiling, Hzymes’ murine RNase inhibitor ensures consistent protection against RNase contamination—even under stressful lab conditions.

Performance Validated Under Real Conditions

Our in-house testing demonstrates that Hzymes' RNase inhibitor remains stable after repeated freeze-thaw cycles and short-term thermal stress, delivering reliable RNase suppression throughout.

This product meets the rigorous expectations of both academic researchers and industrial-scale RNA product developers, helping preserve sample quality and maximize experimental reproducibility.

Thermal Stability and Freeze-thaw Stability Test of Hzymes' RNase inhibitor (Murine) The enzyme activity is not affected after freeze-thaw for 10 times or thermostable when incubate at 55°C for 5 mins.

Why Choose Hzymes?

With over 20 years of enzyme R&D and manufacturing expertise, Hzymes is committed to advancing high-purity, regulatory-compliant raw materials for molecular diagnostics and RNA therapeutics.

Our RNase inhibitor is backed by:

• ISO9001 / ISO13485 certifications

• GMP-compliant production facilities

• Global DMF registration for regulatory support

Conclusion: Safeguard Your RNA with Confidence

RNA degradation due to RNase contamination is avoidable—with the right tools. Hzymes' GMP-grade Murine RNase Inhibitor offers a powerful, stable, and regulatory-compliant solution to protect your valuable RNA samples across diverse applications.

Why Choose Hzymes?

With over 20 years of enzyme R&D and manufacturing expertise, Hzymes is committed to advancing high-purity, regulatory-compliant raw materials for molecular diagnostics and RNA therapeutics.

Our RNase inhibitor is backed by:

• ISO9001 / ISO13485 certifications

• GMP-compliant production facilities

• Global DMF registration for regulatory support

Conclusion: Safeguard Your RNA with Confidence

RNA degradation due to RNase contamination is avoidable—with the right tools. Hzymes' GMP-grade Murine RNase Inhibitor offers a powerful, stable, and regulatory-compliant solution to protect your valuable RNA samples across diverse applications.