Empower Protein Engineering with High-Performance Protease Tools

Protein engineering and biopharmaceutical development rely on precise molecular tools to modify, process, and characterize proteins efficiently. Proteases are essential enzymes widely used for fusion tag removal, recombinant protein purification, antibody fragmentation, and structural analysis.

Hzymes provides a comprehensive portfolio of recombinant protein engineering enzymes, including SUMO Protease, TEV Protease, HRV-3C Protease, and IdeZ Protease. Designed with high specificity, high activity, and excellent stability, these proteases offer reliable solutions for researchers working in recombinant protein expression, antibody drug development, vaccine research, and biopharmaceutical manufacturing.

With optimized enzyme performance and convenient purification features, Hzymes protein engineering proteases help accelerate research workflows and improve experimental efficiency.

Four Key Proteases for Advanced Protein Engineering Applications

Recombinant SUMO Protease: Highly Specific SUMO Fusion Protein Cleavage

SUMO Protease is a recombinant fragment of ULP1 (Ubiquitin-like-specific Protease 1) derived from Saccharomyces cerevisiae and expressed in E. coli. Unlike traditional proteases that recognize linear peptide sequences, SUMO Protease specifically recognizes the tertiary structure of SUMO protein, enabling precise cleavage with minimal nonspecific proteolysis.

Key Features

• Highly specific recognition of SUMO tertiary structure
• Efficient cleavage at the C-terminal region of SUMO fusion proteins
• Excellent activity across a broad range of reaction conditions:
• pH range: 6.0–8.5
• Temperature range: 2–30°C
• NaCl concentration: 0–400 mM
• C-terminal His-tag design for easy protease removal
• Purity ≥95% (SEC-HPLC)

Applications

• Cleavage of SUMO fusion proteins
• Recombinant protein purification workflows
• Production of tag-free target proteins

Enzyme Activity Definition

One unit of enzyme activity is defined as the amount of enzyme required to cleave 5 μg of substrate with a cleavage efficiency greater than 95% within 1 hour at 30°C.

Recombinant TEV Protease: Reliable Fusion Tag Removal Solution

TEV Protease is a recombinant cysteine protease derived from Tobacco Etch Virus (TEV). It is widely used in molecular biology and protein purification due to its high cleavage specificity and ability to remove commonly used fusion tags.

Key Features

• Specifically recognizes the TEV cleavage sequence:

Glu-Asn-Leu-Tyr-Phe-Gln↓Gly/Ser

• Enables precise removal of fusion tags with minimal nonspecific cleavage
• Engineered for improved stability and enzyme activity
• Maintains activity under broad conditions:
• Temperature: 4–34°C
• pH: 6.0–8.5
• C-terminal His-tag design for convenient enzyme removal
• Purity ≥95% (SDS-PAGE)

Applications

• Removal of GST tags
• Removal of MBP tags
• Removal of Trx tags
• Removal of His tags and other fusion tags
• Recombinant protein purification and downstream processing

Enzyme Activity Definition

One unit of enzyme activity is defined as the amount of enzyme required to cleave more than 95% of 3 μg recombinant protein within a 10 μL reaction system after incubation for 1 hour at 30°C.

Recombinant HRV-3C Protease: Low-Temperature Protein Cleavage Tool

HRV-3C Protease is a recombinant 3C protease derived from human rhinovirus 14 and expressed in E. coli. It provides efficient and specific cleavage for fusion protein processing, especially when protein stability is critical.

Key Features

• Specifically recognizes the HRV-3C cleavage sequence:

Leu-Glu-Val-Leu-Phe-Gln↓Gly-Pro

• Active at 4°C, enabling gentle low-temperature protein processing
• Helps maintain target protein activity and structural integrity
• C-terminal His-tag design allows easy removal after cleavage
• Purity ≥95% (SDS-PAGE)

Applications

• Fusion protein tag removal
• Recombinant protein processing
• Protein structure and function studies

Enzyme Activity Definition

Using fusion protein as the substrate, one unit of enzyme activity is defined as the amount of enzyme required to cleave 100 μg of substrate with a cleavage efficiency greater than 95% after incubation for 16 hours at 4°C in a 1× HRV 3C buffer system.

Recombinant IdeZ Protease: Precise IgG Antibody Fragmentation Enzyme

IdeZ Protease, also known as Immunoglobulin G-Degrading Enzyme Z, is a recombinant enzyme derived from Streptococcus equi subsp. zooepidemicus. It specifically cleaves IgG antibodies at the hinge region to generate defined antibody fragments.

Key Features

• Highly specific cleavage of IgG antibodies
• Generates F(ab')₂ and Fc fragments through precise hinge-region cleavage
• C-terminal His-tag design for efficient enzyme removal
• Purity ≥95% (SDS-PAGE)

Applications

• Antibody drug characterization
• Antibody fragment preparation
• Fc and F(ab')₂ fragment generation
• Immunoassay optimization by reducing heavy-chain interference

Enzyme Activity Definition

One unit of enzyme activity is defined as the amount of enzyme required to cleave 1 μg human IgG with a cleavage efficiency greater than 95% within 15 minutes at 37°C in a 10 μL reaction system.

Why Choose Hzymes Protein Engineering Enzymes?

High Specificity for Accurate Protein Processing

Hzymes proteases are engineered for precise substrate recognition, enabling efficient cleavage while minimizing unwanted protein degradation.

High Activity for Improved Research Efficiency

Optimized enzyme performance supports rapid and reliable protein processing, reducing experimental time and improving workflow efficiency.

High Stability for Flexible Applications

Broad reaction compatibility allows researchers to select suitable conditions for different protein engineering applications.

Reliable Recombinant Enzyme Manufacturing

With advanced enzyme engineering platforms and large-scale production capabilities, Hzymes provides high-quality recombinant enzymes for research, diagnostics, and biopharmaceutical applications.

Applications of Protein Engineering Proteases

Hzymes protease products support a wide range of applications, including:

• Recombinant protein expression and purification
• Fusion protein tag removal
• Antibody engineering and antibody drug research
• Protein structural analysis
• Vaccine development research
• Biopharmaceutical process optimization

Explore Hzymes Protein Engineering Enzyme Solutions

From SUMO Protease and TEV Protease for recombinant protein processing to HRV-3C Protease and IdeZ Protease for advanced protein and antibody applications, Hzymes provides versatile enzyme solutions to support modern protein engineering research.

Contact Hzymes to learn more about our recombinant protease products and customized enzyme solutions.