Unlock 2x the Digestion Power with Half the Units—Hzymes GMP-Grade Enzymes Explained

In the development of mRNA-based therapeutics and nucleic acid drugs, precision, safety, and consistency are non-negotiable. A critical step in in vitro transcription (IVT) workflows is the linearization of plasmids before transcription. Hzymes provides GMP-grade restriction enzymes—BsaI and BspQI—engineered to deliver high fidelity, stability, and efficiency for high-stakes applications in nucleic acid drug production.

Why Choose Hzymes GMP-Grade BsaI and BspQI?

Hzymes’ Type IIS restriction enzymes—BsaI and BspQI—are purpose-built for IVT template preparation. These enzymes are free from animal-origin components (AOF) and produced under strict GMP-grade conditions, ensuring the highest level of safety and compliance for pharmaceutical manufacturing. They are ideal for applications where no additional sequence incorporation is required, providing clean and reliable plasmid linearization.

Key Features
✅ Fast Digestion & High Efficiency: Complete digestion with significantly fewer enzyme units than leading competitors.
✅ High Thermal Stability: Maintains full activity even after extended incubation at 37°C.
✅ Exceptional Fidelity & Specificity: Designed for precise cleavage with minimal off-target activity.
✅ GMP-Grade & Animal-Origin-Free: Manufactured under GMP-compliant conditions and suitable for clinical and commercial drug production.
✅ FDA DMF Certificate Ready: Supports regulatory submissions and quality assurance requirements.

Performance

BsaI Digestion Efficiency

• With 3 cleavage sites: Only 10 U of Hzymes BsaI is needed to digest 10 µg plasmid at 37°C in 2 hours, whereas other suppliers require over 20 U.

• With 1 cleavage site: Only 5–10 U of Hzymes BsaI completes digestion, compared to more than 10 U from competitors.

BspQI Digestion Efficiency

• For plasmids with a single BspQI site, just 1.25 U of Hzymes BspQI is sufficient to digest 5 µg of plasmid at 50°C in 1 hour—outperforming competitors who need over 5 U.

Stability at Elevated Temperatures

BsaI Stability Test

BsaI retained full enzyme activity after incubation at 37°C for 5 days, showing no significant degradation compared to Supplier A’s enzyme.

BspQI Stability Test

Likewise, BspQI remained highly stable with consistent activity after 7 days at 37°C, proving its robustness for prolonged storage and reaction conditions.

GMP-Grade Restriction Enzymes You Can Trust

With the global demand for high-quality mRNA and gene therapies continuing to rise, reliable restriction enzymes are more important than ever. Hzymes BsaI and BspQI stand out as optimal tools for pharmaceutical-grade plasmid preparation, thanks to their efficiency, stability, and regulatory readiness.

Whether you’re working on preclinical studies or full-scale commercial production, Hzymes offers restriction enzymes that meet the rigorous demands of modern biotechnology.

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