mRNA Synthesis
Hzymes possesses the necessary restriction endonucleases for the preparation of linearized plasmid templates, enzyme raw materials for in vitro transcription, modified nucleosides, cap analogues, purification, and quality control materials. It can provide customized synthesis of mRNA with high yield, purity, and cost-effectiveness in different specifications.

Hzymes provides full process services from sequence design to mRNA synthesis, supporting the synthesis of various forms of RNA such as linear mRNA, saRNA, CircRNA, etc. It also has a professional quality control platform, which can better safeguard the research and development of RNA related products for customers.

Technical process

Self-Amplifying RNA
Self-amplifying RNA (saRNA) is an mRNA that encodes a replicase and can self-replicate within cells using itself as a template. SaRNA contains the basic elements of mRNA (cap, 5’UTR, 3’UTR, and variable length polyadenylation tail), with a large open reading frame (ORF) at the 5 ‘end encoding four nonstructural proteins (nsP1-4) and a sub genome promoter to facilitate RNA self-replication. The self-replication property of saRNA can achieve lower dosages of RNA drugs, prolong protein expression time in vivo, and reduce side effects.

Hzymes can provide customized synthesis services for saRNA, selecting the optimal expression elements, sequence design, and base modification to ensure efficient in vivo and in vitro expression of saRNA, and better assist in the research and development of saRNA products.

The delivery efficiency of saRNA EGFP under low-dose conditions is higher than that of mRNA, showing a significant dose advantage

The expression persistence of self-replicating saRNA has a significant advantage in persistence

CircRNA
Circular RNA (circRNA) is a novel non coding RNA produced by reverse splicing of pre mRNA precursors. Unlike traditional linear RNA, circRNA has a closed circular structure formed by covalent bonds at the 5 ‘and 3’ ends, which is not affected by RNA nucleases, resulting in more stable expression, less degradation, and lower immunogenicity.

Hzymes can provide customized synthesis services for CircRNAs, supporting the synthesis of circular RNAs of different fragment lengths, ensuring efficient expression of CircRNAs in vivo and in vitro, and better assisting in RNA therapies such as Car-T and protein replacement in vivo, as well as the research and development of RNA related products such as gene regulation and drug delivery.

Service Process

Delivery Standard

Customized synthesis of CircRNA

Tools for RNA Finished Products
In mRNA drug research, it is often necessary to use ready-made mRNA raw materials as cell or animal experimental controls, or to explore purification processes, screen fillers, optimize mRNA expression levels, and evaluate carrier delivery efficiency.

Hzymes provides a variety of IVT mRNA raw materials in stock, including COVID-19, EGPF encoding, Fluc or mCherry reporter genes, DNA endonuclease gene Cas9, erythropoietin gene EPO precursor, etc. Linear mRNA and saRNA forms can be selected to provide more assistance for mRNA research.

Product features:

CASE
Can produce high-purity mRNA of different lengths

Multiple testing items for mRNA raw material can be provided