Intended Use
For the in vitro quantitative determination of α-Amylase (AMY) levels in human serum and plasma.
It is mainly used for the auxiliary diagnosis and treatment of acute pancreatitis (inflammation of the pancreas).
Principle
4, 6-ethylene-p-nitrobenzene-α-D-maltohept oside (ethylene-G7-pNP, EPS) is decomposed by α-Amylase, then the degradation products are hydrolyzed to p-nitrophenol (405 nm) under α-glucosidase. The color of p-nitrophenol formed is proportional to the activity of α-amylase.
Reagents
|
α-AMY |
Composition |
Concentration |
|
Reagent 1 (R1) |
||
|
HEPES |
50 mmol/L |
|
|
NaCl |
70 mmol/L |
|
|
MgCl2 |
10 mmol/L |
|
|
α-glucosidase |
≥4 KU/L |
|
|
Reagent 2(R2) |
||
|
EPS |
3.5 mmol/L |
|
Storage and Stability
12 months from production when stored at 2–8°C from light and sealed package.
Once opened, store the reagents protected from light at 2–8°C.
Do not freeze the reagents.
