Product Overview

Alkaline phosphatases are a group of non-specific hydrolytic enzymes with high catalytic activity under alkaline conditions, capable of catalyzing the hydrolysis of almost all phosphomonoesterases to orthophosphate and its counterparts such as alcohols, phenols and sugars. Alkaline phosphatase from bovine small intestine is a homodimer with high enzyme activity and good thermal stability. Suitable for various immunological applications, including chemiluminescence, ELISA, Western blot and immunohistochemistry.

Key Features

High Specific Activity

Exhibits high enzymatic activity under alkaline conditions, supporting efficient signal generation across immunoassay applications.

Sensitive Signal Detection

Provides a high signal-to-background ratio with low background luminescence, making it suitable for assays requiring sensitive detection.

Good Stability

Demonstrates stable performance under storage and accelerated stability conditions, supporting consistent assay results over time.

Consistent Batch Performance

Manufactured under controlled production processes to ensure batch-to-batch consistency, with variation maintained within established quality specifications.

Applications

Chemiluminescent Immunoassays

Suitable for use in chemiluminescent detection platforms requiring alkaline phosphatase-based signal amplification.

ELISA

Applicable to enzyme-linked immunosorbent assays (ELISA) for antigen and antibody detection.

Western Blotting

Can be used in Western blot detection workflows utilizing alkaline phosphatase-conjugated reagents.

Immunohistochemistry (IHC)

Suitable for immunohistochemical staining applications requiring enzymatic signal development.

Performance Data

High specific activity: The specific activity of ALP from Hzymes across three batches is higher than that of brand R

High signal-to-noise ratio, lower background luminescence and stable clinical performance

Excellent accelerated thermal stability with higher signal retention rate

The product features good stability, with inter-assay CV controlled within ±10%

FAQ

What applications is this product suitable for?

This product is suitable for a variety of immunoassay applications, including chemiluminescent immunoassays, ELISA, Western blotting, and immunohistochemistry (IHC).

What are the recommended storage conditions and shelf life of this product?

The product is recommended to be stored at 2–8°C under the specified storage conditions. Please refer to the product specification sheet for the validated shelf life.

Do you provide OEM or private-label manufacturing services?

Yes. We offer OEM and private-label manufacturing services to support customers' specific product and branding requirements.

What are the shipping conditions and typical lead times?

The product is typically shipped under cold-chain conditions (2–8°C).

In stock: lead time of approximately 2–3 weeks

Out of stock: lead time of approximately 8–12 weeks

How is batch-to-batch consistency controlled?

The product is manufactured under established quality control procedures to ensure consistent performance across production batches, supporting reliable assay development and manufacturing.