Product Overview

Alkaline phosphatases are a group of non-specific hydrolytic enzymes with high catalytic activity under alkaline conditions, capable of catalyzing the hydrolysis of almost all phosphomonoesterases to orthophosphate and its counterparts such as alcohols, phenols and sugars. Alkaline phosphatase from bovine small intestine is a homodimer with high enzyme activity and good thermal stability. Suitable for various immunological applications, including chemiluminescence, ELISA, Western blot and immunohistochemistry.

Key Features

High Specific Activity

Exhibits high enzymatic activity under alkaline conditions, supporting efficient signal generation in immunoassay applications.

Sensitive Signal Detection

Provides a high signal-to-background ratio with low background luminescence, enabling reliable detection across a range of assay formats.

Good Stability

Demonstrates stable performance under recommended storage and accelerated stability conditions, supporting consistent assay performance over time.

Consistent Batch Performance

Manufactured under controlled production processes to ensure batch-to-batch consistency, with variation maintained within established quality specifications.

Hypoglycosylated Format

Produced as a hypoglycosylated alkaline phosphatase, providing an alternative enzyme format for customers evaluating different assay development requirements.

If you have data demonstrating advantages related to reduced glycosylation (e.g., conjugation efficiency or assay optimization), this section can be further refined accordingly.

Applications

Chemiluminescent Immunoassays

Suitable for use in chemiluminescent detection platforms utilizing alkaline phosphatase-based signal generation.

ELISA

Applicable to enzyme-linked immunosorbent assays (ELISA) for antigen and antibody detection.

Western Blotting

Can be used in Western blot applications employing alkaline phosphatase-conjugated detection reagents.

Immunohistochemistry (IHC)

Suitable for immunohistochemical staining workflows requiring enzymatic signal development.

Performance Data

High Specific Activity for Enhanced Detection Performance

ALP from <br />Hzymes demonstrates higher specific activity than Brand R across three independent batches, ensuring superior catalytic performance.

High Signal-to-Noise Ratio with Low Background and Stable Clinical Performance

Delivers strong signal intensity with reduced background luminescence, enabling improved assay sensitivity and reliable clinical performance. <br />

Excellent Thermal Stability for Long-Term Signal Preservation

Maintains excellent stability under accelerated thermal stress conditions, with a high signal retention rate after testing. <br />

High Detection Repeatability and Batch-to-Batch Consistency

Provides consistent assay performance with inter-assay CV controlled within ±10%.<br />

FAQ

What applications is this product suitable for?

This product is suitable for a variety of immunoassay applications, including chemiluminescent immunoassays, ELISA, Western blotting, and immunohistochemistry (IHC).

What are the recommended storage conditions and shelf life of this product?

The product should be stored at 2–8°C under the recommended storage conditions. Please refer to the product specification sheet for validated shelf-life information.

Do you provide OEM or private-label manufacturing services?

Yes. We offer OEM and private-label manufacturing services to support customers' formulation, packaging, and branding requirements.

What are the shipping conditions and typical lead times?

The product is typically shipped under cold-chain conditions (2–8°C) to maintain product quality.

In stock: Typical lead time is 2–3 weeks.

Out of stock: Typical lead time is 8–12 weeks.

How is batch-to-batch consistency controlled?

The product is manufactured under established quality control procedures to ensure consistent performance between batches, with batch-to-batch variation maintained within defined quality specifications.