Product Overview

RNase contamination is a major risk factor in RNA-based molecular diagnostic workflows, particularly in RT-PCR and RT-qPCR assays. Even trace RNase activity can rapidly degrade RNA templates, leading to reduced sensitivity and unreliable results.

Hzymes Glycerol-Free RNase Inhibitor is a recombinant murine RNase inhibitor expressed in E. coli, specifically designed for molecular diagnostic applications that require glycerol-free enzyme systems. It binds tightly to RNase A, B, and C in a 1:1 non-covalent complex, effectively blocking RNase activity and preserving RNA integrity during downstream reactions.

The glycerol-free formulation is optimized for applications where glycerol may interfere with assay performance, lyophilization processes, or reagent system integration in diagnostic kit development.

Key Features

Glycerol-Free Formulation

Suitable for lyophilized diagnostic reagents and systems where glycerol compatibility is restricted or undesirable.

Strong RNase Inhibition Activity

Effectively inhibits RNase A, B, and C to protect RNA integrity in sensitive molecular diagnostic assays.

High Stability

Maintains consistent activity under routine handling conditions, including freeze-thaw cycles and short-term temperature exposure.

Oxidation-Resistant Murine Design

Enhanced resistance to oxidative inactivation compared with human or porcine RNase inhibitors, supporting reliable performance in low-DTT systems.

High Purity for Diagnostic Use

Manufactured under strict quality control with low levels of host cell DNA, host cell protein, endotoxin, and nuclease residues.

Applications

• RT-PCR and RT-qPCR-based molecular diagnostics

• RNA sample protection in clinical diagnostic workflows

• Lyophilized (freeze-dried) diagnostic reagent systems

• Multiplex and automated molecular diagnostic platforms

• RNA handling steps in in vitro diagnostic (IVD) kit development

Performance Data

RNase Inhibition Efficiency

Effectively inhibits RNase A, B, and C through stable 1:1 binding, ensuring RNA stability in RT-PCR and RT-qPCR systems.

Freeze-Thaw Stability

Maintains enzymatic activity after multiple freeze-thaw cycles, supporting routine diagnostic reagent preparation and storage conditions.

Compatibility Performance

Demonstrates stable performance in molecular diagnostic reaction systems, including glycerol-sensitive and lyophilized formulations.

FAQ

Q1: What is the advantage of glycerol-free RNase inhibitor?

A: It is optimized for lyophilized and dry diagnostic systems without affecting enzyme stability.

Q2: Is performance comparable to glycerol-containing versions?

A: Yes. It maintains equivalent RNase inhibition activity.

Q3: What applications is it designed for?

A: Lyophilized RT-PCR kits, POCT systems, and automated diagnostic platforms.

Q4: Does it improve room-temperature stability?

A: It improves compatibility with dry formulations and enhances formulation flexibility.