Sample Release Reagent (Colorimetric LAMP)

The Sample Release Reagent (Colorimetric LAMP) is a molecular POCT sample preparation solution designed for rapid, extraction-free nucleic acid detection. It enables direct amplification of crude lysates from clinical swab samples, supporting fast and efficient LAMP colorimetric detection workflows.

By eliminating conventional nucleic acid extraction steps, this reagent significantly reduces sample-to-result time while maintaining detection performance comparable to standard extraction-based methods. It is optimized for nasal swabs, throat swabs, and other respiratory sample types, making it suitable for decentralized testing and point-of-care molecular diagnostics.

Product Overview

The Sample Release Reagent (Colorimetric LAMP) is developed for extraction-free molecular diagnostics workflows based on LAMP technology. It rapidly lyses swab samples and releases nucleic acids directly into a form compatible with downstream colorimetric LAMP reactions.

After treatment, the sample can be directly added to LAMP amplification systems (e.g., HMD5204, HMD5205, HMD5206) without further purification. This streamlined workflow supports fast pathogen detection and simplifies operational steps in clinical and field environments.

The reagent is designed to meet the requirements of molecular POCT applications, where speed, simplicity, and reliability are essential.

Key Features

Extraction-Free Workflow

Enables direct amplification from crude swab lysates, eliminating the need for nucleic acid extraction and reducing hands-on time.

Optimized for Colorimetric LAMP Systems

Specifically designed for compatibility with colorimetric LAMP detection platforms, supporting visible endpoint detection in addition to simplified instrumentation.

Broad Sample Compatibility

Suitable for a range of respiratory clinical samples, including:

• Nasal swabs

• Throat swabs

• Other swab-based specimen types

Fast Sample-to-Result Time

Accelerates molecular testing workflows by enabling immediate downstream amplification after sample processing.

High Analytical Consistency

Processed samples deliver detection performance comparable to conventional nucleic acid extraction-based workflows.

POCT-Friendly Design

Supports decentralized testing scenarios with minimal equipment requirements and simplified operation procedures.

Flexible Processing Conditions

Recommended minimum lysis volume is approximately 400 μL per swab, adjustable based on assay requirements.

Applications

The Sample Release Reagent (Colorimetric LAMP) is widely used in rapid molecular diagnostics and POCT workflows, including:

• Respiratory pathogen detection using swab samples

• Point-of-care testing (POCT) in clinical settings

• Rapid screening in emergency or field diagnostics

• LAMP-based infectious disease testing

• Decentralized laboratory and low-resource environments

• Colorimetric LAMP diagnostic assays for visual readout systems

Note:

After sample processing, downstream testing should be performed promptly. The recommended holding time before LAMP reaction is less than 1 hour to ensure optimal performance.

FAQ

Q1: What is special about the colorimetric LAMP sample release reagent?

A: It is optimized for colorimetric LAMP systems, enabling direct use of crude samples while maintaining clear visual readout performance.

Q2: Does it interfere with color change detection?

A: No. It is designed to minimize interference with pH- or dye-based colorimetric LAMP readouts.

Q3: What sample types can it be used with?

A: It is suitable for nasal swabs, throat swabs, and other clinical crude samples.

Q4: Does it support extraction-free workflows?

A: Yes. It is specifically designed for extraction-free molecular diagnostics.

Q5: Can it be used with fluorescent LAMP systems?

A: It is optimized for colorimetric systems; compatibility with fluorescent systems requires validation.