Product Overview

Cas9 mRNA (modified) encodes the Streptococcus pyogenes Cas9 (SpCas9) protein, a key nuclease used in CRISPR-Cas9 genome editing technologies. By combining with sequence-specific sgRNA, the translated Cas9 protein recognizes targeted genomic DNA sequences and generates double-strand DNA breaks (DSBs), enabling precise genome modification.

This modified Cas9 mRNA incorporates N1-methylpseudouridine (N1-Me-pUTP) modification, a commonly used nucleoside modification strategy that can improve mRNA stability, enhance translation efficiency, and reduce innate immune activation. The encoded Cas9 protein contains NLS sequences at both N- and C-termini to promote nuclear localization, as well as a C-terminal HA epitope tag for convenient protein detection and purification.

Produced through in vitro co-transcriptional capping and purified by precipitation and desalting, Cas9 mRNA (modified) provides sustained protein expression in mammalian cells. With a theoretical length of 4522 nt, it is designed for advanced CRISPR genome editing research, cellular engineering, and in vivo studies.

Amino Acid Sequence of Encoded Protein：

MAPKKKRKVGIHGVPAADKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKNLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQIGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQLGGDSGGKRPAATKKAGQAKKKKGSYPYDVPDYA*

Key Features

N1-Me-pUTP Modified mRNA for Enhanced Performance

Incorporates N1-methylpseudouridine modification to improve mRNA stability, translation efficiency, and cellular compatibility.

Efficient SpCas9 Protein Expression

Enables robust expression of functional Cas9 nuclease for CRISPR-mediated genome editing applications.

Reduced Innate Immune Recognition

Modified nucleosides help minimize unwanted immune responses associated with exogenous mRNA delivery.

Optimized Nuclear Localization

Dual NLS sequences facilitate nuclear transport of Cas9 protein and support efficient DNA cleavage.

Reliable Stability and Flexible Supply

• Stable for 1 month at 2–8°C

• More than 10 freeze-thaw cycles tolerated

• Available with customization options and reliable production capacity

Applications

Cas9 mRNA (modified) is suitable for applications requiring efficient and stable Cas9 protein expression, including:

• CRISPR-Cas9 genome editing

• Gene knockout and knock-in studies

• Primary cell and mammalian cell engineering

• Functional genomics screening

• Stem cell and regenerative medicine research

• In vivo gene editing studies

• Development and evaluation of genome editing delivery systems

Performance Data

Western Blot Analysis of Cas9 Protein Expression in 293T Cells

Stability of mRNA Bulk Solution at 2–8°C

The modified Cas9 mRNA maintains excellent stability during refrigerated storage.

Freeze-Thaw Stability

The product retains mRNA integrity and translation activity after repeated freeze-thaw cycles.

Long-Term Stability at -80°C

Cas9 mRNA (modified) shows reliable long-term stability under frozen storage conditions.

FAQ

Q1: What is the difference between Cas9 mRNA and Cas9 mRNA (modified)?

Cas9 mRNA (modified) contains N1-methylpseudouridine (N1-Me-pUTP) modification, which can enhance translation efficiency, improve mRNA stability, and reduce innate immune activation compared with unmodified Cas9 mRNA.

Q2: What modification is used in Cas9 mRNA (modified)?

The product incorporates N1-methylpseudouridine (N1-Me-pUTP), a widely used modification in therapeutic and research mRNA applications.

Q3: What are the advantages of using Cas9 mRNA instead of Cas9 protein?

Cas9 mRNA enables transient intracellular expression of Cas9 protein after delivery, avoiding the need for direct protein delivery and supporting flexible genome editing workflows.

Q4: How should Cas9 mRNA (modified) be stored and transported?

Recommended storage temperature is -85°C to -65°C. Transportation is supported under -45°C to 25°C conditions.

Q5: Is customized Cas9 mRNA available?

Yes. Sequence optimization, modification selection, and customized production services are available.

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