Product Overview

Recombinant Inorganic Pyrophosphatase (Yeast) is a high-purity pyrophosphate hydrolysis enzyme produced from engineered E. coli expressing the Saccharomyces cerevisiae ppa gene. Designed for molecular biology research and biopharmaceutical manufacturing workflows, this enzyme efficiently removes inorganic pyrophosphate (PPi) accumulation to enhance reaction efficiency and product yield.

During nucleic acid synthesis processes, including in vitro transcription (IVT), PCR amplification, and DNA/RNA synthesis, accumulated PPi can inhibit enzymatic reactions and limit final product output. Inorganic Pyrophosphatase catalyzes the conversion of PPi into orthophosphate (Pi), shifting reaction equilibrium toward product formation and improving synthesis performance.

With excellent activity, purity, and stability, Hzymes Yeast-derived Inorganic Pyrophosphatase is suitable for high-yield mRNA production, gene therapy development, and advanced molecular biology applications, supporting both research-scale and GMP manufacturing workflows.

Key Features

GMP-Ready Quality

• Animal origin-free and free of β-lactam antibiotics

• Manufactured under stringent quality control standards with high batch-to-batch consistency

• Supported by DMF filing for biopharmaceutical applications

High Purity and Low Residual Impurities

• Protein purity ≥99%

• Nuclease-free with low residual HCD, HCP, microorganisms, metal ions, and endotoxin levels

Superior Stability

• Excellent thermal stability and freeze-thaw stability

• Extended shelf life compared with imported counterparts

High Enzyme Activity

• High catalytic activity for efficient PPi removal

• Superior activity performance compared with peer products under equivalent conditions

Enhanced Reaction Performance

• Effectively reduces PPi-mediated inhibition

• Improves yield and efficiency in IVT, amplification, and nucleic acid synthesis systems

Applications

Hzymes Inorganic Pyrophosphatase (Yeast) is widely used in reaction systems where inorganic pyrophosphate accumulation affects synthesis efficiency and product yield.

Key applications include:

mRNA In Vitro Transcription (IVT)

Enhances transcription efficiency and improves mRNA yield by reducing PPi inhibition

PCR and Isothermal Amplification

Supports efficient nucleic acid amplification by maintaining reaction activity

DNA/RNA Synthesis

Improves synthesis efficiency in enzymatic nucleic acid production workflows

mRNA Vaccines and Gene Therapy Development

Suitable for preclinical research and GMP-scale manufacturing processes

Performance Data

High Enzyme Activity Compared with Competitors

Hzymes Yeast-derived Inorganic Pyrophosphatase demonstrates higher enzyme activity across multiple batches compared with competitor products, ensuring consistent and efficient PPi hydrolysis performance.

Excellent Thermal Stability

Accelerated stability testing at 37°C shows strong residual activity retention over time, indicating superior thermal stability and reliable performance during manufacturing workflows.

Enhanced Freeze-Thaw Stability

After multiple freeze-thaw cycles, the enzyme maintains high residual activity, supporting stable storage and transportation conditions.

Residual activity after incubation at 37°C for different durations

FAQ

Q1: What is Inorganic Pyrophosphatase used for?

Inorganic Pyrophosphatase hydrolyzes inorganic pyrophosphate (PPi) into orthophosphate (Pi). By removing PPi accumulation, it reduces reaction inhibition and improves the efficiency and yield of nucleic acid synthesis processes, including IVT and amplification reactions.

Q2: What are the main applications of Yeast-derived Inorganic Pyrophosphatase?

It is widely used in mRNA in vitro transcription, PCR, isothermal amplification, DNA/RNA synthesis, and other enzymatic reaction systems requiring efficient PPi removal.

Q3: Is this product suitable for GMP manufacturing?

Yes. The enzyme is produced with GMP-oriented quality standards, is animal origin-free, free of β-lactam antibiotics, and supported by DMF filing, making it suitable for biopharmaceutical process development and manufacturing applications.

Q4: How should the product be stored and transported?

Transport under 0°C and store at -25°C to -15°C. Avoid repeated freeze-thaw cycles to maintain optimal enzyme activity.

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