Yeast Inorganic Pyrophosphatase
Inorganic pyrophosphatase (PPase) catalyzes the hydrolysis of inorganic pyrophosphate to generate orthophosphate to eliminate the inhibition of inorganic pyrophosphate to the reaction system. Inorganic pyrophosphatase is widely used in molecular biology to improve the production of RNA molecules, especially for in reverse transcription reaction and in vitro transcription of mRNA.

Features
• High enzyme activity and high purity: the enzyme activity is higher than other supplier and the protein purity is as high as 99%.
• Excellent catalyze effect of hzymes inorganic pyrophosphate.
• Super thermal stability and freeze-thaw stability.
• Low impurity and strict QC panel: low HCD/HCP/ metal ion/endotoxin residue; no nulcease residue.
• GMP-Grade, Non-Animal Origin; DMF Record: MF036853.

Performance

Gel analysis of mRNA yield after IVT with different brands of Inorganic Pyrophosphatase (yeast)  Hzymes' Inorganic pyrophosphatase (yeast) significantly improve the yield of mRNA compare to other competitors.

Higher enzyme activity of Haymes' Inorganic Pyrophosphatase compare to other suppliers and high stability among batches.

Thermal Stability validation. Higher residual activity of Hzymes' inorganic pyrophosphatase after incubation at 37°C compare to other suppliers and high stability among batches.

Product Description

Pyrophosphatase Inorganic (yeast) is derived from a recombinant E. coli strain carrying Saccharomyces cerevisiae ppa gene. The enzyme catalyzes the hydrolysis of inorganic pyrophosphate to form orthophosphate. P20₇ ^–4 + H₂0 → 2HP0₄ ^–2 Pyrophosphatase Inorganic (yeast) could hydrolyze the inorganic pyrophosphate generated by nucleic acid amplification, in vitro transcription and other reactions, removing the inhibition of the inorganic pyrophosphate generated on the reaction system. The removal of pyrophosphate can shift the reaction equilibrium to the end of product formation, which is beneficial to increase the yield of synthetic product. RNA and DNA synthesis are examples of reactions that can be pulled far in the synthesis direction by the action of inorganic pyrophosphatase.

Product Information

Storage

Store at -25～-15 ℃, valid for 2 years ( Avoid repeated freeze-thaw cycles ).

Unit definition

One unit is the amount of enzyme that will generate 1 μmol of phosphate per minute from inorganic pyrophosphate under standard reaction conditions.
Standard reaction conditions: a 10-minute reaction at 25°C in 100 mM Tris-HCl, [pH 7. 2], 2 mM MgCl₂ and 2 mM PPi in a reaction volume of 0. 5 ml.