Enzyme Mix 2.0

Protease K |Creatinase lamp |dNTP |All-purpose nuclease mRNA

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Enzyme Mix 2.0

1 mL: HBP000332-2

10mL: HBP000332-3

100mL: HBP000332-4

Cat. No: HBP000332

Transportation and storage: Transportation under 0°C and storage at -25～- 15 °C.

Technical Support

Enzyme mix 2. 0 contain T7 RNA polymerase (M6)、RNase Inhibitor、Pyrophosphatase Inorganic (yeast), Suitable for co-transcription capping systems.

1300nt		2000nt		3000nt		4000nt
Yield (μg)	Integrity (%)	Yield (μg)	Integrity (%)	Yield (μg)	Integrity (%)	Yield (μg)	Integrity (%)
190. 8	87. 6%	267. 4	89. 9%	182. 4	92. 0%	216. 3	87. 6%

Product Description

Enzyme Mix 2. 0 is an enzyme mixture containing T7 RNA polymerase, inorganic pyrophosphatase (yeast), and RNase inhibitors. It reduces the number of reagent addition steps during the preparation of in vitro transcription reagents, facilitating the operation of in vitro transcription reactions. This reagent is used together with NTPs, in vitro transcription buffer, and DNA templates containing the T7 promoter. A single reaction can yield 150-200 μg of RNA products and can be scaled up to produce milligram quantities of RNA. The transcribed RNA can be used for applications including RNA structure and function research, RNase protection, probe hybridization, anti-sense RNA, and RNAi.

The production and quality management procedures for this product comply with GMP standards, ensuring traceability of the production process and all raw and auxiliary materials. The entire production process does not use ampicillin or any animal-derived raw materials. This product strictly controls residues of endonuclease, exonuclease, nickase, RNase, and endotoxins.