Product Overview

Mouse IL-12 mRNA (modified) encodes murine interleukin-12 (IL-12), a multifunctional cytokine that plays a central role in immune regulation and antitumor immunity. IL-12 promotes Th1 immune responses by inducing Th0 cell differentiation into Th1 cells, enhancing Th1 cell proliferation, and stimulating IFN-γ secretion from NK cells and T cells.

Through activation of NK cells, LAK cells, and cytotoxic T lymphocytes (CTLs), IL-12 contributes to enhanced cellular immune responses and has been extensively studied in cancer immunotherapy and immune engineering research.

This product contains optimized murine IL-12b and IL-12a coding sequences connected by a peptide linker. A miR-122 binding site is introduced into the 3' UTR to suppress IL-12 expression in hepatocytes while preserving expression in tumor cells, supporting targeted IL-12 expression studies.

Mouse IL-12 protein expression can be confirmed by Western blot (WB) and ELISA analysis.

Mouse IL-12 mRNA (modified) is synthesized by in vitro co-transcriptional capping with N1-methylpseudouridine (N1-Me-pUTP) modification and purified through precipitation and desalting. The N1-methylpseudouridine modification reduces innate immune activation and improves mRNA translation performance, making it suitable for advanced mRNA delivery and immunotherapy research.

Amino Acid Sequence of Encoded Protein：

MCPQKLTISWFAIVLLVSPLMAMWELEKDVYVVEVDWTPDAPGETVNLTCDTPEEDDITWTSDQRHGVIGSGKTLTITVKEFLDAGQYTCHKGGETLSHSHLLLHKKENGIWSTEILKNFKNKTFLKCEAPNYSGRFTCSWLVQRNMDLKFNIKSSSSSPDSRAVTCGMASLSAEKVTLDQRDYEKYSVSCQEDVTCPTAEETLPIELALEARQQNKYENYSTSFFIRDIIKPDPPKNLQMKPLKNSQVEVSWEYPDSWSTPHSYFSLKFFVRIQRKKEKMKETEEGCNQKGAFLVEKTSTEVQCKGGNVCVQAQDRYYNSSCSKWACVPCRVRSGGGGSGGGGSGGGGSRVIPVSGPARCLSQSRNLLKTTDDMVKTAREKLKHYSCTAEDIDHEDITRDQTSTLKTCLPLELHKNESCLATRETSSTTRGSCLPPQKTSLMMTLCLGSIYEDLKMYQTEFQAINAALQNHNHQQIILDKGMLVAIDELMQSLNHNGETLRQKPPVGEADPYRVKMKLCILLHAFSTRVVTINRVMGYLSSA*

Key Features

Reduced Innate Immune Activation

N1-methylpseudouridine modification helps reduce mRNA immunogenicity and improves compatibility for in vivo studies.

Enhanced Translation Efficiency

Modified nucleosides support efficient protein production in mammalian eukaryotic cells.

Tumor-Oriented IL-12 Expression Design

The engineered 3' UTR containing a miR-122 binding site helps regulate IL-12 expression patterns between hepatocytes and tumor cells.

Excellent Stability and Quality

Stable for at least 1 month at 2–8°C.

Resistant to more than 10 freeze-thaw cycles.

Suitable for long-term storage at -80°C.

Validated Functional Expression

IL-12 protein expression can be confirmed by WB and ELISA assays.

Applications

Mouse IL-12 mRNA (modified) is suitable for:

• Cancer immunotherapy research

• mRNA-based cytokine therapy development

• Tumor microenvironment studies

• Immune cell activation studies

• NK cell and T cell functional analysis

• In vivo mouse model evaluation

• mRNA delivery system development

Performance Data

Cellular Expression Assay

Demonstrates efficient IL-12 protein expression after transfection into mammalian cells.

Stability of mRNA Bulk Solution at 2–8°C

Maintains product stability under refrigerated conditions.

Freeze-Thaw Stability

Maintains integrity and activity after repeated freeze-thaw cycles.

Long-Term Stability at -80°C

Supports long-term storage with stable product performance.

FAQ

Q1: What modification is included in Mouse IL-12 mRNA (modified)?

A: The product incorporates N1-methylpseudouridine (N1-Me-pUTP) modification during mRNA synthesis.

Q2: What is the advantage of N1-methylpseudouridine modification?

A: N1-methylpseudouridine modification can reduce innate immune recognition and improve mRNA translation efficiency.

Q3: How can IL-12 expression be measured?

A: IL-12 protein expression can be detected by Western blot and ELISA assays.

Q4: What are the recommended storage conditions?

A: Store at -85°C to -65°C. Transportation is supported under -45°C to 25°C conditions.

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