RTL reverse transcriptase 2.0 is an RNA template-dependent DNA polymerase that lacks the 3' → 5' exonuclease activity and has RNase H activity. This enzyme can use RNA as a template to synthesize a complementary strand of DNA, which can be applied to first-strand cDNA synthesis, especially for RT-LAMP (loop-mediated isothermal amplification). Compared with RTL reverse transcriptase 1.0, the sensitivity is significantly improved, the thermal stability is stronger, and the reaction at 65°C is more stable. RTL reverse transcriptase 2.0 (glycerol free) can be used to prepare freeze-dried preparations, freeze-dried RT-LAMP reagents, etc.
| Product Name | Cat. No | Size |
| RTL Reverse Transcriptase 2.0 (Glycerol-free) | HMD5302-01 | 1500U |
| HMD5302-02 | 15000U | |
| HMD5302-03 | 150000U |
Precautions
1）This product will form a white solid when stored at -20 °C. Take it out from -20°C and put it on ice for about 10 minutes. After melting, it can be used by shaking and mixing.
2）The cDNA product could be stored at -20°C or -80°C or used immediately for PCR reaction.
3）To prevent RNase contamination, please keep the experimental area clean, and wear clean gloves and masks during operation.
