Preparation and specification
| Appearance | Yellow amorphous powder, lyophilized |
| Purity(SDS-PAGE) | ≥90% |
| Enzyme powder specific activity | ≥600.0 U/mg |
| Glucose-6-phosphate dehydrogenase | ≤0.01% |
| NADH/NADPH oxidase | ≤0.01% |
Properties
| EC number | 1.6.5.2 (Recombinant from microorganism) | |
| Molecular weight | 28 kDa (SDS-PAGE) | |
| Isoelectric point | 5.2 | |
| Michaelis constants | 7.4 ×10-4 M (NADH), 6.0×10-2 M (NADPH) | |
| Inhibitors | Fe3+,Mn2+,Cu2+ | |
| Optimum pH | 7.5 | Fig.1 |
| Optimum temperature | 45℃ | Fig.2 |
| pH stability | pH 4.5-9.0 (25 ℃, 16 h) | Fig.3 |
| Thermal stability | Below 50 ℃ (pH 7.5, 30 min) | Fig.4 |
| Storage stability | At least one year at -25～-15 ℃ | Fig.5 |
| Stabilizers | FMN，NAD(P)H | |
Applications
This enzyme is useful for colorimetric determination of NAD(P)H and many dehydrogenases when coupled with various dyes which act as hydrogen acceptors from NAD(P)H.
Unit definition
One unit (U) is defined as the amount of enzyme which consumes 1 μmol of DCPIP per min under the conditions described below.
