Product Description
Hi-yield T7 in vitro transcription reagent (Trace dsRNA) is designed for in vitro transcription of
RNA with low dsRNA byproduct using T7 RNA
polymerase mutant with optimizing transcription
reaction system. The kit is capable of synthesizing mRNA using linear double-stranded DNA containing T7 promoter as template and NTPs as substrate. The kit is
suitable for incorporation of modified
nucleotides to obtain biotin labeled, dye labeled
and radiolabeled RNA. The kit also can be
applied in synthesis of co-transcriptionally
capping mRNAs with cap analogs. The kit contains modified nucleotides N1-Me-pUTP as alternative.
Each standard reaction yields up to 150-200 μg
of RNA from 1μg DNA template. Reaction size
can be scaled up to yield milligram-level RNA
as needed.
RNA synthesized from the kit is suitable for many applications including RNA structure and
function studies, ribozyme biochemistry, RNase protection assays and hybridization-based blots, anti-sense RNA and RNAi experiment. RNA
synthesized from the kit also is suitable for producing enzymatic production of capped RNA
by vaccinia capping enzyme (HBP000606) and
2’-O-Methyltransferase (HBP000701) and being
tailed up with Poly(A) polymerase (HBP000801)
to improve the stability and translational competence of RNA used for in vitro translation, transfection, and microinjection.
Product Information
| Product Name | Cat. No |
| Hi-yield T7 in vitro transcription reagent (Trace dsRNA) | HBP001516 |
Transportation and storage
Transportation under 0°C and storage at -25～ -15°C.
