Product Overview

Hi-yield T7 in vitro transcription reagent kit is designed for in vitro transcription of RNA using T7 RNA polymerase. The kit is capable of synthesizing high yield RNA transcripts using linear double-stranded DNA containing T7 promoter as template and NTPs as substrate.

The kit is suitable for incorporation of modified nucleotides to obtain biotin labeled, dye labeled and radiolabeled RNA. The kit also can be applied in synthesis of co-transcriptionally capping mRNAs with cap analogs. The kit contains modified nucleotides N1-Me-pUTP as alternative.

Each standard reaction yields up to 150-200 μg of RNA from 1μg DNA template. Reaction size can be scaled up to yield milligram-level RNA as needed.

RNA synthesized from the kit is suitable for many applications including RNA structure and function studies, ribozyme biochemistry, RNase protection assays and hybridization-based blots, anti-sense RNA and RNAi experiment.

RNA synthesized from the kit also is suitable for enzymatic production of capped RNA by vaccinia capping enzyme (HBP000606) and 2’-O-Methyltransferase (HBP000701), and for polyadenylation with Poly(A) polymerase (HBP000801) to improve the stability and translational competence of RNA used for in vitro translation, transfection, and microinjection.

Key Features

High-Yield RNA Synthesis

Generates up to 180-220 μg of RNA from 1 μg of DNA template under standard reaction conditions, enabling efficient RNA production and scalable manufacturing.

Enhanced RNA Integrity

Powered by an engineered high-specific-activity T7 RNA Polymerase, the kit delivers transcripts with excellent integrity while minimizing transcriptional side reactions, supporting reliable downstream performance.

Broad Compatibility

Compatible with a wide range of modified nucleotides and cap analogs, including N1-Me-pUTP, enabling the synthesis of diverse RNA modalities for research and therapeutic applications.

Flexible Template Support

Efficiently transcribes templates ranging from 200 bp to 12 kb, accommodating RNA products of varying lengths and complexities.

Ready-to-Use IVT Workflow

Provided as a complete, pre-optimized IVT system containing all essential reagents, eliminating manual formulation and reducing workflow setup time.

Applications

mRNA Vaccine and Therapeutic Development

Suitable for the generation of high-quality IVT products for the development of mRNA vaccines, gene therapies, and other RNA-based therapeutics.

Modified mRNA Production

Supports the incorporation of modified nucleotides such as N1-Me-pUTP, as well as co-transcriptional capping using cap analogs, enabling the production of modified mRNAs with enhanced functionality.

High-Quality RNA Synthesis

The kit incorporates an engineered high-specific-activity T7 RNA Polymerase mutant (Cat. No. HBP000330), delivering significantly improved transcription efficiency and reducing undesired side reactions to enhance both RNA yield and integrity.

Accelerated IVT Workflow Development

As a complete, ready-to-use transcription system, the kit streamlines process setup and minimizes optimization efforts, allowing users to rapidly obtain reproducible, high-quality IVT products.

Performance Data

Yield Comparison & Integrity Comparison

dsRNA Content Comparison

Performance on Different Length Templates

Component List

FAQ

Is this kit compatible with N1-Me-pUTP?

Yes. The kit supports the incorporation of N1-Me-pUTP and other modified nucleotides for modified mRNA synthesis.

Can capped mRNA be generated using this kit?

Yes. The kit is compatible with co-transcriptional capping using cap analogs.

What RNA yield can be achieved?

Up to 150-200 μg of RNA can be generated from 1 μg of DNA template, with scalable reactions for larger production needs.

What applications is this kit suitable for?

The kit is suitable for modified mRNA production, mRNA vaccine research, gene therapy development, and other IVT applications requiring high-yield RNA synthesis.

How should the kit be stored and transported?

Transport below 0°C and store at -25 to -15°C.