Product Overview

Streptavidin-Alkaline Phosphatase Conjugate (SA-ALP) is obtained by crosslinking Streptavidin (SA) and Alkaline Phosphatatase (ALP). It is applicable for the detection of biotinylated samples including antibodies, proteins, tissues, cells, and nucleic acids. Often used as a signal amplifier to enhance detection sensitivity, it is widely employed in molecular biology, biochemistry, immunology, and other related fields.

This product uses recombinant Streptavidin (SA) with a molecular weight of approximately 53.2 kDa. Structurally, it removes sequences unrelated to activity, retaining only the active core sequences. It outperforms natural SA in stability, solubility, and other aspects. Existing as a homotetramer, each mole of the tetramer can bind to four moles of biotin molecules. This alkaline phosphatase, recombinantly expressed in Pichia pastoris, features high purity, high activity and other excellent characteristics.

Key Features

Controlled batch-to-batch consistency

Variation across three consecutive batches is controlled within ±10% under standard testing conditions.

Comparable performance with reference products

Luminescence signal levels of calibrators are generally comparable with commonly used reference-grade reagents under validated assay conditions.

Good stability

Maintains over 90% signal retention after a 7-day accelerated stability test at 37 °C under defined conditions.

Broad assay compatibility

Suitable for multiple biotin–streptavidin-based detection platforms, including CLIA, ELISA, Western Blot, flow cytometry, immunohistochemistry, in situ hybridization, and protein microarray applications.

Applications

Chemiluminescence immunoassay (CLIA)

Used as an enzyme conjugate in biotin–streptavidin systems to support signal generation and detection in chemiluminescent immunoassays.

ELISA (enzyme-linked immunosorbent assay)

Applicable in streptavidin-based ELISA workflows for detection of biotinylated antibodies or antigens using ALP-mediated signal development.

Western Blot (WB)

Used for detection of biotinylated proteins or probes on membranes, supporting colorimetric or chemiluminescent readout systems.

Flow Cytometry (FC)

Applied in biotin-labeled antibody detection systems for analysis of cell surface or intracellular targets.

Immunohistochemistry (IHC)

Suitable for tissue-based detection of biotinylated targets using ALP-mediated signal visualization.

In Situ Hybridization (ISH)

Used in nucleic acid detection workflows with biotin-labeled probes in histological samples.

Protein microarray

Applicable in high-throughput protein interaction and expression analysis based on biotin–streptavidin detection systems.

Performance Data

Batch-to-batch variation of three batches is controlled within ±10%

Ultra-low non-specific adsorption

NC membrane (nitrocellulose membrane) for HPV in situ hybridization (ISH) molecular detection

Signal retention rate remains above 90% after 7-day accelerated testing at 37 °C

FAQ

What are the recommended storage conditions and shelf life of this product?

Store at 2–8 °C. Do not freeze. Keep the product tightly sealed and avoid prolonged exposure to ambient conditions.

What is the recommended usage or dosage for this product?

Recommended working dilutions vary depending on the application:

• Western Blot (WB): 1:4,000–1:12,000

• ELISA: 1:5,000–1:20,000

• IHC/ICC: 1:500–2,000

• EMSA: 1:5,000–1:15,000

• CLIA: 1:20,000–1:50,000
Optimization may be required based on specific experimental conditions.

Do you provide OEM or private-label manufacturing services?

Yes, OEM and private-label services are available upon request.

What are the shipping conditions and typical lead times?

For in-stock products, typical lead time is 2–3 weeks. For out-of-stock items, lead time is approximately 8–12 weeks depending on production scheduling and order volume.