Product Overview
D-Lactic dehydrogenase (DLDH) catalyzes the reversible conversion of pyruvate and NADH into D-Lactic acid and NAD+, exhibiting specificity for D-Lactic acid without cross-interference from L-lactic acid. It is a key enzymatic component in IVD reagents. This product is manufactured via high-density microbial fermentation followed by column chromatographic purification, produced on an industrial scale with a protein purity ≥90% and a specific activity of ≥420 U/mg in lyophilized form. Impurities are tightly controlled, batch-to-batch consistency is excellent, and long-term stable supply is ensured. Its unique advantages include a pyruvate affinity 2-3 orders of magnitude higher than that of competing products, broad pH stability from 5.0 to 9.0, and outstanding thermal stability. DLDH (HH1701) enhances the performance of HCY (cystathionine-based) and ALT/GPT assays, while also being well suited for assays targeting D-Lactic acid in bacterial infection typing, as well as metabolites such as pyruvate, ATP, glucose, and glycerol. The product complies with global IVD regulatory standards and is available in customized specifications and technical services, with reliable supply to customers worldwide.
Key Features
Excellent substrate specificity
HH1701 does not react with L-lactic acid in the blood, improving the accuracy of detection.
Extraordinary stability
Incubation at 50°C for 30 minutes maintains enzyme activity at over 90%. Incubation at 37°C for one week preserves dry-powder activity at over 90%. Storage at−20°C for one year also ensures that dry-powder activity remains above 90%. These characteristics provide robust stability for the development of diagnostic reagents targeting ALT, AST, and HCY.
Excellent inter-batch stability
The difference between batches is minimal, ensuring the stability of reagent batches during use.
Greater specific activity, and excellent stability
Compared with competing products, it exhibits a lower Km, an affinity for the substrate that is 2-3 orders of magnitude higher, greater specific activity, and excellent stability.
| Test Items | HH1701 | VentorA | VentorB |
|---|---|---|---|
| Specific activity(U/mg) | ≥420 | ≥400 | ≥150 |
| Michaelis constants(Pyruvate) | 2.2×10-6M | 1.6×10-4M | 1.2×10-3M |
| Inhibitors | Ag+, Hg2+ | Ag+, Hg2+ | / |
| Optimum pH | 6.5-7.0 | 6.0-7.0 | 7.0 |
| Optimum temperature | 45°C | 35-40°C | / |
| pH stability | 5.5-10.0 | 5.0-9.0 | 4.0-10.0 |
| Thermal stability | ≤50°C | ≤45°C | ≤50°C |
| 37°C, residual activity after 7days | 100.75% | 92.66% | / |
| 49°C Half-life (min) | 1275.1 | / | / |
Applications
D-LDH (HH1701), when used in conjunction with relevant enzymes, can be employed for the development and large-scale preparation of assay reagents targeting a variety of metabolites-including pyruvate, D-Lactic acid, HCY, ATP, ADP, glucose, creatinine, and glycerol-as well as enzymes such as alanine aminotransferase (ALT), pyruvate kinase (PK), and creatine kinase (CK).
Reaction Principle
The amount of NADH can be measured at 340nm using a spectrophotometer. One unit of enzyme activity is defined as the amount of enzyme required to oxidize 1μmol of NADH per minute under conditions of pH7.7 and 37°C.
Specification
| CAS | 9028-36-8 |
|---|---|
| EC Number | EC 1.1.1.28 |
| Appearance | White amorphous lyophilized powder |
| Dry powder specific activity | ≥420.0U/mg |
| Protein Purity | ≥90%(SDS-PAGE) |
| Protectants | BSA |
| Contaminants | NADH/NADPH oxidase≤0.01%; Malate dehydrogenase≤0.005%; Glutamate dehydrogenase≤0.003%; Pyruvate kinase≤0.03% |
| Optimal pH | pH6.5-7.0 |
| pH stability | pH5.5-10.0 (25°C, 16 h) |
| Optimal temperature | 45°C |
| Km | 2.2×10-6M (Pyruvate) |
| Temperature stability | Below 50°C (pH7.0, 30 min) |
| Long-term stability | Stored at -20°C for 12 months, maintaining over 90% activity. |
| MW | 57kDa(SDS-PAGE) |
| pI | 4.5 |
| Inhibitors | Ag+, Hg2+ |
Available Formats
• HH1701-01 10kU
• HH1701-02 100kU
• HH1701-03 500kU
• HH1701-04 1000kU
• HH1701-99 Customizable
Performance Data
Excellent correlation
The alanine aminotransferase/serine aminotransferase (ALT/GPT) reagent from Hzymes demonstrates excellent correlation with results obtained using reagents from leading manufacturers in clinical samples (R² > 0.99), with an average bias of less than ±10%.
Excellent stability
Hzymes' alanine aminotransferase/serine aminotransferase (ALT/GPT) reagents exhibit excellent stability; after 7 days of accelerated testing at 37°C, their performance remains as stable as at the start.
Application of HH1701 in HCY (cystathionine method) reagents
The assay's sensitivity, accuracy, and repeatability all meet the required specifications.
| Test Items | Specification | Result | Conclusion |
|---|---|---|---|
| Reagent appearance |
R1: colorless clear liquid, no visible flocculent substances or precipitates are observed R2: light yellow clear liquid, no visible flocculent substances or precipitates are observed |
R1: colorless clear liquid, no visible flocculent substances or precipitates are observed R2: light yellow clear liquid, no visible flocculent substances or precipitates are observed |
PASS |
| pH | R1: 9.00±0.1, R2: 6.50±0.1 | R1: 8.97, R2: 6.58 | PASS |
| Reagent blank absorbance | ≥0.8 | 1.5617 | PASS |
| Sensitivity | △A≥0.01 | 0.0113 | PASS |
| Accuracy | ≤±15%(Quality control) | Quality control 1: 0.28%, Quality control 2: 0.65% |
PASS |
| Repeatability | Quality control 1:≤5%, Quality control 2: ≤3% | Quality control 1: 0.69%, Quality control 2: 0.68% |
PASS |
| Stability (37°C 7days) |
≤±10% | Quality control 1: 4.67%, Quality control 2: 2.86% |
PASS |
Wide linear range
Within the HCY concentration range of 0-50μmol/L, the kit exhibits excellent assay linearity.
Good correlation
When testing 80 samples, the assay results showed good correlation with those obtained using the reference reagent, with a bias of less than 5%.
Excellent stability
stored sealed at 4°C, it remains stable for 18 months; once opened, it can be stably stored for 30 days.
