Multiplex qPCR Probe Mix U+ (Fast)

Protease K |Creatinase lamp |dNTP |All-purpose nuclease mRNA

Home · Products · Products · Molecular Diagnostics · PCR Master Mix · DNA Mix · Multiplex qPCR Probe Mix U+ (Fast) ·

100 rxns: HMD6801-01

1,000 rxns: HMD6801-02

10,000 rxns: HMD6801-03

Cat. No: HMD6801

Storage condition: Storage at -25℃ ~ -15℃ for 1 year. Transport with ice packs.

Technical Support

Product description

Multiplex qPCR Probe Mix U+ (Fast) is designed for Real-Time qPCR using probe detection. This mix can complete 42 cycles of amplification within 40 minutes. 50×Enzyme mix U+ contains heat-labile Uracil N-Glycosylase, which degrades amplification products from the previous reaction. This mix can be used for multi-color fluorescence melting curve analysis.

Product components

Component	100 rxns	1,000 rxns	10,000 rxns
4×qPCR buffer U+	1.25mL	12.5mL	125mL
50×Enzyme mix U+	100μL	1mL	10mL

Notes:

a. 4×qPCR buffer U+ includes dNTP and Mg²⁺;

b. 50× Enzyme mix U+ includes Hot-Start Taq DNA polymerase and UDG.

Transportation and storage

Storage at -25℃ ~ -15℃ for 1 year. Transport with ice packs.

IMPORTANT

Before use, thoroughly mix the 4×qPCR buffer U+. If there is any precipitation after thawing, wait for the buffer to return to room temperature, mix and dissolve, and then use them normally.

Reaction Setup

Component	50 μL reaction
4×qPCR buffer U+	12.5μL
50× Enzyme mix U+	1μL
Primer Forward	0.1~1.0μM
Primer Reverse	0.1~1.0μM
TaqMan Probe	0.05~0.2μM
DNA	X μL
dd H₂O	Up to 50μL

Cycling protocol: Standard

Step	Cycles	Temperature	Duration (min:sec)
UDG incubation	1	37℃	02:00
Initial denaturation	1	95℃	00:30
Denaturation	45	95℃	00:10
Annealing&Extension	45	60℃	00:30

Cycling protocol: Fast

Step	Cycles	Temperature	Duration (min:sec)
UDG incubation	1	37℃	02:00
Initial denaturation	1	95℃	00:30
Denaturation	42	95℃	00:05
Annealing&Extension	42	60℃	00:10

Notes: The optimal annealing temperature needs to be adjusted based on the Tm value of the primer, and select the shortest time for fluorescence signal collection based on the Real Time PCR instrument.