Product Overview

PNGase F is the most efficient enzymatic method to remove nearly all N-glycans from glycoproteins. PNGaseF digests the glycosidic bond between the innermost N-acetylglucosamine and asparagine residues. It cleaves N-glycans attached to glycoproteins, including high-mannose, hybrid, and complex oligosaccharide glycoforms.

Key Features

Efficient N-glycan release enzyme

PNGase F-Fast enables enzymatic removal of N-linked glycans from glycoproteins by cleaving the bond between the innermost N-acetylglucosamine and asparagine residues. It is applicable to high-mannose, hybrid, and complex N-glycan structures.

Preservation of glycan core structure

After enzymatic digestion, the released N-glycans retain their core structures, supporting downstream analytical applications.

Rapid reaction setup

Supports fast deglycosylation workflows with a typical setup time of approximately 10 minutes at 50 °C under recommended conditions.

High purity

Purity is typically ≥95% as determined by standard analytical methods.

Flexible reaction conditions

Compatible with both denaturing and non-denaturing digestion workflows depending on experimental requirements.

Multiple product formats available

Different enzyme formats are available to support diverse application needs (e.g., HBP003013 /HBP003004/HBP003005).

Applications

Rapid N-glycan release for analysis

Used for enzymatic removal of N-linked glycans in workflows requiring reduced digestion time, typically incubated at 50 °C under optimized conditions.

CE and chromatography-based glycan analysis

Suitable for sample preparation in capillary electrophoresis (CE), HPLC, and LC-MS-based N-glycan profiling workflows.

Glycoprotein modification for downstream applications

Applied for removal of N-glycans prior to downstream processes such as protein modification and bioconjugation (e.g., ADC-related workflows), depending on experimental design.

Performance Data

Enzymatic digestion efficiency

FAQ

What components are included in the PNGase F-Fast system?

The PNGase F-Fast system typically includes PNGase F-Fast enzyme and 5×PNGaseF-Fast buffer, providing a simplified setup for rapid N-linked glycan release workflows.

Can this system be used for both denaturing and non-denaturing conditions?

Yes. The PNGase F-Fast system is compatible with both denaturing and non-denaturing reaction conditions. The choice of workflow depends on the sample type and experimental requirements.

What are the recommended reaction conditions for PNGase F-Fast?

Under recommended conditions, the reaction can be set up in approximately 10 minutes at 50 °C, followed by incubation according to the specific workflow and sample type to achieve efficient N-glycan release.

Can the reaction conditions be optimized?

Yes. Enzyme amount, incubation time, and reaction conditions can be adjusted based on glycoprotein type, glycan complexity, and downstream analytical requirements.

Protocols under denaturing and non-denaturing conditions