The Hot start Plus Taq DNA Polymerase (Antibody modified) is a rapid amplification Taq DNA polymerase developed by our company, which tightly blocks 5’→3’ polymerase activity and 5’→3’ exonuclease activity at room temperature. This product utilizes the Whole-Block Taq antibody V1 (HMD0321) to block both the 5’→3’ polymerase activity and 5’ →3’ exonuclease activity, making it suitable for use in pre-mixed reagents with multiple primers and probes. It can prevent nonspecific amplification, as well as the loss of nonspecific signals and amplification efficiency due to degradation of primers, probes, or other critical components, thereby ensuring the stability of amplification reagents on two fronts. This product can be rapidly activated by heating at 95°C for 30 seconds, which fully releases the activity. It is suitable for fast PCR or multiplex quantitative PCR detection using DNA as the template. This product supports pre-mixing of primers and probes. With high specificity and amplification sensitivity, it is compatible with rapid protocols, reducing detection time.
Product Information
Product Name | Cat. No | Size |
Hot Start Plus Taq DNA Polymerase (Antibody modified) (5 U/uL) | HMD0210-01 | 1 KU |
HMD0210-02 | 10 KU | |
HMD0210-03 | 100 KU |
One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into DNA in 30 minutes at 72°C with M13 mp18 Single Strand DNA (Virion DNA) as template / primer.