Applications 

In vitro test for the quantitative determinatio n of HbA1c concentration in human whole blood on photometric systems. HbA1c is a p roduct of hemoglobin (Hb) that produces a s low and continuous non-enzymatic glycation reaction under high blood glucose. Glucose modifies hemoglobin specifically in its n-ter minal valine residue to form glycated hemog lobin. Under normal physiological conditions, the production of non-enzymatic glycosylati on reaction products is positively proportiona l to the concentration of reactants. Since he moglobin concentration remains relatively stable, glycosylation levels are mainly dependen t on glucose concentration and are also relat ed to the length of hemoglobin and glucose exposure. Therefore, HbA1c is a good indica tor to the average blood glucose level of pat ients for the last 2~3 months.

Principle 

Under the action of protease, the n-terminal of β chain in HbA1c is cut off and glycosylated dipeptides are released. In the first reaction, Hb concentration can be obtained by measuring the absorbance of 480 nm. In the second reaction, fructosyl peptide oxidase (FPOX) acts on glycosylated dipeptides to generate hydrogen peroxide which can react with chromogenic agents to generate a absorbance at 660nm in the presence of peroxidase, then the concentration of HbA1c can be obtained by measuring the absorbance of 660nm. According to the obtained HbA1c concentration and Hb concentration, the percentage of HbA1c(HbA1c%) can be calculated.

Storage and stability

12 months from manufacturation when stored at 2-8℃ in the dark environment and sealed package.
Once opened, the reagents are stable for 28 days when refrigerated on the analyzer or refrigerator. Contamination of the reagents must be avoided. Do not freeze the reagents.

Attention 

Reagents with different batch numbers cannot be mixed. Please calibrate again when changing the reagent batch number!