Applications 

Used for quantitative determination of glycated albumin (GA) and albumin (ALB) in human serum，the ratio of glycated albumin (%) is used glycated albumin concentration divided by the albumin concentration.
Glycated albumin is a non-enzymatic reaction between glucose and albumin in the blood, because the half-time of albumin in vivo is short (about 17-19 days）, so GA serves as a 2-3 week indicator of average blood glucose. Therefore，it has significant clinical application value When blood sugar needs to be evaluated for short-term control.

Principle 

1.Determination of glycated albumin In the sample (serum), specific proteases are added to decompose Glycated albumin to form glycosylated amino acids. Then glycosylated amino acids are converted into glucoaldosterone, amino acids, and hydrogen peroxide by specific glycosylated amino acid oxidase. Under the action of peroxidase, hydrogen peroxide reacts with 4-amino-aminoantipyrine and TOOS to form red quinone-imine[3]. The concentration of glycated albumin in the sample was obtained by measuring its absorbance.

2.Determination of albumin In pH 4.2, the albumin in the sample (serum) binds with the indicator bromocresol green to form a blue-green complex. The concentration of albumin in the sample was obtained by measuring the change of absorbance.

3. Calculation of glycated albumin value (%)  The albumin reagent originated from Hzymes.

Storage and stability 

Up to expiration date indicated on the label, when stored unopened at 2-8℃ and protected from light.
Once opened, the reagents are stable for 30 days when refrigerated on the analyzer or refrigerator. Contamination of the reagents must be avoided. Do not freeze the reagents.
Once dissolved, the calibrator are stable for 7 days at 2-8℃, the control are stable for 7 days at2-8℃，do not freeze.

Warnings and precautions 

1.For in vitro diagnostic use.
2.Take the necessary precautions for the use of laboratory reagents.
3.Preservative contained. Do not swallow. Avoid contact with skin and mucous membranes.
4.Disposal of all waste material should be in accordance with local guidelines.
5.Material safety data sheet is available on request for professional users.