Product Description
Proteinase K is a stable serine protease with broad substrate specificity. It degrades many proteins in the native state even in the presence of detergents. Evidence from crystal and molecular structure studies indicates the enzyme belongs to the subtilisin family with an active site catalytic triad (Asp39-His69-Ser224). The predominant site of cleavage is the peptide bond adjacent to the carboxyl group of aliphatic and aromatic amino acids with blocked alpha amino groups. It is commonly used for its broad specificity.
Product Information
| Product Name | Cat. No | Size |
| Proteinase K (lyophilized powder) | HH4500-01 | 100 mg |
| HH4500-02 | 1 g | |
| HH4500-03 | 10g | |
| HH4500-04 | 100g | |
| HH4500-99 | Customized size |
Specification
| Appearance | White to off-white amorphous powder, lyophilized |
| Activity | ≥30 U/mg |
| DNase | None detected |
| RNase | None detected |
| Nickase | None detected |
Properties
| Properties | ||
| EC number | 3.4.21.64(Recombinantfrom Tritirachiumalbum) | |
| Molecular weight | 29kDa (SDS-PAGE) | |
| Isoelectric point | 7.81 | |
| Optimum pH | 7.0-12.0 | Fig.1 |
| Optimum temperature | 65℃ | Fig.2 |
| pH stability | pH 4.5-12.5 (25℃,16h) | Fig.3 |
| Thermal stability | Below50℃ (pH8.0,30min) | Fig.4 |
| Storage stability | Over 90% activity stored at 25℃ for 12months | Fig.5 |
| Activators | SDS,urea | |
| Inhibitors | Diisopropylfluorophosphate; phenylmethylsulfonylfluoride | |
| Storage condition | Dry powder condition ≤8℃ valid for 36 months | |
Applications
Genetic diagnostic kit; RNA and DNA extraction kits; Extraction of non-protein components from tissues, degradation of protein impurities, such as DNA vaccines and sodium acetate, 0.3 M acetic acid. Reagent III: 0.4 M Na2CO3 solution. Reagent IV: Forint reagent diluted with pure water for 5 times. Reagent V: enzyme diluent: 0.1 M sodium phosphate solution (pH 8.0). Reagent VI: tyrosine solution: 0, 0.005, 0.025, 0.05, 0.075, 0.1, 0.25 umol/ml tyrosine dissolved with 0.2 M HCl.
Precautions
Wear protective gloves and goggles when using or weighing, and keep well ventilated after use. This product may cause skin allergic reaction. Cause serious eye irritation. If inhaled, it may cause allergy or asthma symptoms or dyspnea. May cause respiratory irritation
Unit definition
One unit (U) is defined as the amount of enzyme required to hydrolyze casein to produce 1 μmol tyrosine per minute under the following conditions.
