mRNA Cap 2'-O-methyltransferase is an essential enzyme for the enzymatic capping process, which can add a methyl group to cap 0 structure and forming the Cap 1 structure.

Features
• GMP-grade: no animal origin; DMF record: 036852.
• Low residue: no nuclease residue, HCD, HCP, microorganism, metal ion, endotoxin residue is low.
• Good stability: the thermal stability is better than the imported products.
• High enzyme activity and high purity: the enzyme activity is higher than similar competing products, and the protein purity is as high as 99%.

  Performance

After the optimization of enzymatic capping reaction system, CAP1 accounted for 99%.

Figure Spectrophotometry determination of radioactive phosphor, P32.
(Left)Thin layer chromatography (TLC) of non-radioactive standards.
(Right) Spectrophotometry determination of radioactive phosphor, P32, showing that the the substrate has been basically all modified into cap0 and cap1 structure after the optimization of enzymatic reaction system.

Product Description

mRNA Cap 2´ -O-methyltransferase was derived from a recombinant E. coli strain that carries the gene for the vaccinia mRNA Cap 2´-O-Methyltransferase. This enzyme adds a methyl group at the 2´-O position of the first nucleotide adjacent to the cap structure at the 5´ end of the RNA. The enzyme utilizes S-adenosylmethionine (SAM) as a methyl donor to methylate capped RNA (cap-0) resulting in a cap-1 structure.

The Cap 1 structure can increase the translation efficiency, improving the expression of mRNA in transfection and microinjection experiments.
This enzyme specifically requires RNA with an m7GpppN cap as substrate. It cannot utilize RNA with pN, ppN, pppN or GpppN at the 5´ end. Capped RNA may be prepared via in vitro transcription using cap analog or through enzymatic capping using the Vaccinia Capping Enzyme.

Product Information

Shipping and Storage

Stored at -25 °C to -15°C ( Avoid repeated freeze-thaw cycles ).

Unit Definition

One unit is defined as the amount of enzyme required to methylate 10 pmoles of 80 nt capped RNA transcript in 1 hour at 37°C.