Product Overview

Mouse erythropoietin (mouse EPO) is a glycoprotein hormone that is structurally and functionally homologous to human EPO. It plays a critical role in regulating erythropoiesis by promoting the survival, proliferation, and differentiation of erythroid precursor cells.

Mouse EPO mRNA (modified) encodes mouse erythropoietin protein and is designed for efficient protein expression in mammalian cells and animal models. It is widely used in biomedical research related to anemia, erythropoiesis regulation, tumor biology, cardiovascular research, and evaluation of novel therapeutic strategies.

This product is an mRNA synthesized through in vitro co-transcriptional capping and incorporates N1-methylpseudouridine (N1-Me-pUTP) modification to reduce innate immune activation and improve translation efficiency. The mRNA is purified through precipitation and desalting processes, with a theoretical length of 845 nt.

Mouse EPO mRNA (modified) enables stable expression of functional mouse EPO protein in mammalian systems and serves as a valuable tool for studying erythropoietin signaling pathways, physiological mechanisms, and drug discovery applications.

Amino Acid Sequence of Encoded Protein:

MGVPERPTLLLLLSLLLIPLGLPVLCAPPRLICDSRVLERYILEAKEAENVTMGCAEGPRLSENITVPDTKVNFYAWKRMEVEEQAIEVWQGLSLLSEAILQAQALLANSSQPPETLQLHIDKAISGLRSLTSLLRVLGAQKELMSPPDTTPPAPLRTLTVDTFCKLFRVYANFLRGKLKLYTGEVCRRGDR*

Key Features

Enhanced Translation Efficiency

N1-methylpseudouridine modification improves mRNA translation performance and supports robust mouse EPO protein expression in mammalian cells.

Reduced Innate Immune Activation

Modified nucleosides help reduce cellular immune recognition, making the product suitable for sensitive in vitro and in vivo research applications.

Excellent Stability

• Stable for 1 month at 2–8℃

• Maintains integrity after more than 10 freeze-thaw cycles

Validated Cellular Expression Performance

Demonstrates strong protein expression capability in mammalian cell-based assays with higher expression levels compared with competing products.

Flexible Supply & Customization

• Short lead time

• Reliable batch-to-batch consistency

• Custom sequence and formulation services available

Applications

Mouse EPO mRNA (modified) is suitable for:

Erythropoiesis and anemia research

Investigation of erythroid cell development and EPO signaling pathways

Study of mechanisms underlying anemia-related diseases

Cell-based expression assays

Evaluation of mRNA translation efficiency

Protein expression and functional validation studies

In vivo animal studies

Mouse model research related to erythropoietin function

Evaluation of therapeutic approaches targeting erythropoiesis

Drug discovery and screening

Screening of erythropoiesis regulators

Assessment of compounds affecting EPO pathways

Performance Data

Detection of Mouse EPO mRNA (modified) Protein Expression by Western Blot Analysis

Stability of mRNA Bulk Solution at 2–8℃

The product maintains stability during refrigerated storage, providing flexibility for laboratory workflows.

Stability Under Freeze-Thaw Cycles

Mouse EPO mRNA (modified) maintains mRNA integrity and performance after repeated freeze-thaw cycles.

Long-term Stability of mRNA Bulk Solution at -80℃

The product exhibits excellent long-term stability under frozen storage conditions.

FAQ

Q1: What modification is used in Mouse EPO mRNA (modified)?

A: The product contains N1-methylpseudouridine (N1-Me-pUTP) modification, which improves translation efficiency and reduces innate immune activation.

Q2: What is the storage condition for Mouse EPO mRNA (modified)?

A: The recommended storage temperature is -85℃ to -65℃. Transportation conditions range from -45℃ to 25℃.

Q3: Can Mouse EPO mRNA (modified) be used for in vivo studies?

A: Yes. It is suitable for animal model studies and evaluation of erythropoietin-related biological mechanisms.

Q4: Is customization available?

A: Yes. Custom sequence design, modification options, and formulation services are available upon request.

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