Product Overview

Fluc mRNA (modified) is a chemically modified messenger RNA encoding firefly luciferase, designed for enhanced translation performance and reduced innate immune activation in mammalian cells.

By incorporating N1-methylpseudouridine (N1-Me-pUTP) into the RNA sequence, this modified FLuc mRNA improves cellular translation efficiency and supports stronger protein expression compared with conventional unmodified mRNA.

Following transfection, the encoded firefly luciferase protein catalyzes luciferin oxidation to generate bioluminescent signals, enabling rapid and quantitative assessment of mRNA delivery, translation activity, and expression kinetics.

Fluc mRNA (modified) is synthesized through in vitro co-transcriptional capping technology, followed by precipitation and desalting purification. With a theoretical length of 1,908 nt, it provides stable and efficient protein expression in mammalian eukaryotic cells.

Amino Acid Sequence of Encoded Protein：

MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDAHIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAPANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQSMYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHARDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYKIQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGYGLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMSGYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESILLQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVVFVDEVPKGLTGKLDARKIREILIKAKKGGKIAV*

Key Features

Enhanced Translation Efficiency with N1-Me-pUTP Modification

N1-methylpseudouridine modification improves mRNA translation performance and helps reduce recognition by innate immune pathways.

Strong Bioluminescent Reporter Performance

Firefly luciferase expression enables highly sensitive and quantitative detection for evaluating mRNA functionality.

Improved Cellular Compatibility

Modified mRNA supports efficient protein production with reduced cellular immune stimulation compared with unmodified RNA.

Excellent Stability

• Stable for 1 month at 2–8°C

• Tolerates more than 10 freeze-thaw cycles

• Maintains high-quality RNA integrity during storage and handling

Reliable Supply and Customization

• Optimized manufacturing process

• Short production lead time

• Custom sequence and modification services available

Applications

Fluc mRNA (modified) is suitable for:

Evaluation of modified mRNA delivery systems

LNP formulation screening and optimization

mRNA vaccine and therapeutic research

Assessment of modified mRNA translation efficiency and expression duration

Protein expression studies

Quantitative analysis of intracellular protein production

Reporter gene assays

Monitoring gene regulation, signaling pathways, and promoter activity

In vivo expression analysis

Evaluation of biodistribution, protein localization, and delivery efficiency

Performance Data

Protein Expression at Different Time Points

Modified Fluc mRNA enables enhanced and sustained luciferase expression, supporting long-term monitoring of translation activity.

Expression Performance Across Cell Lines

Fluc mRNA (modified) exhibits reliable protein expression across multiple mammalian cell models.

In Vivo Bioluminescence Imaging of mRNA Expression Over Time

mRNA Bulk Solution Stability Testing

Storage stability at 2–8°C

Freeze-thaw cycle tolerance

Long-term stability at -80°C

FAQ

Q1: What is Fluc mRNA (modified)?

Fluc mRNA (modified) is a firefly luciferase reporter mRNA incorporating N1-methylpseudouridine (N1-Me-pUTP) modification to enhance translation efficiency and improve cellular compatibility.

Q2: What is the advantage of N1-Me-pUTP modification?

N1-Me-pUTP modification can increase mRNA translation efficiency, improve protein expression levels, and reduce innate immune activation caused by exogenous RNA.

Q3: What applications is modified Fluc mRNA suitable for?

It is widely used for mRNA delivery evaluation, LNP optimization, protein expression studies, reporter assays, and in vivo expression analysis.

Q4: What are the storage conditions?

The recommended storage condition is -85°C to -65°C, with transportation under -45°C to 25°C.

Q5: Can different modifications or sequences be customized?

Yes. Customized mRNA sequences, modifications, and production services are available according to research requirements.

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