Product Overview

Fluc mRNA is an in vitro transcribed messenger RNA encoding firefly luciferase (FLuc), a highly sensitive bioluminescent reporter protein widely used for monitoring gene expression, protein translation, and delivery efficiency.

After transfection into cells, Fluc mRNA is translated into firefly luciferase, which catalyzes the oxidation of luciferin to generate measurable bioluminescent signals. The simple, rapid, and highly quantifiable detection makes FLuc mRNA an ideal reporter system for evaluating mRNA delivery performance, gene regulation, cellular processes, and in vivo expression profiles.

This product is synthesized through in vitro co-transcriptional capping technology and purified by precipitation and desalting processes. With a theoretical length of 1,908 nt, Fluc mRNA provides efficient translation activity and reliable expression performance in mammalian eukaryotic cells.

The encoded firefly luciferase protein sequence is derived from Photinus pyralis and enables convenient luminescence-based detection without the need for complex imaging procedures.

Amino Acid Sequence of Encoded Protein：

MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDAHIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAPANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQSMYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHARDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYKIQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGYGLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMSGYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESILLQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVVFVDEVPKGLTGKLDARKIREILIKAKKGGKIAV*

Key Features

High Sensitivity Bioluminescent Reporter System

Firefly luciferase generates strong bioluminescent signals through luciferin oxidation, enabling sensitive and quantitative analysis of protein expression.

Efficient Protein Expression

Fluc mRNA delivers robust translation performance after transfection, enabling reliable evaluation of mRNA expression efficiency and delivery systems.

Excellent Stability

• Stable for 1 month at 2–8°C

• Resistant to more than 10 freeze-thaw cycles

• Maintains RNA integrity during routine handling and storage

Ready-to-Use and Customizable

• Produced with optimized in vitro transcription processes

• Available with flexible customization options

• Short lead time and reliable supply capacity

Applications

Fluc mRNA is suitable for a wide range of research applications, including:

mRNA delivery system evaluation

Screening and optimization of lipid nanoparticles (LNPs) and other delivery platforms

Gene expression analysis

Quantitative monitoring of mRNA translation efficiency and protein expression levels

Reporter gene assays

Functional studies of promoters, regulatory elements, and signaling pathways

Cell differentiation and biological process studies

Tracking cellular responses and molecular regulation

In vivo protein expression studies

Evaluation of tissue distribution, protein localization, and delivery efficiency in animal models

Performance Data

Protein Expression at Different Time Points

Fluc mRNA enables sustained luciferase expression over multiple time points, supporting dynamic monitoring of mRNA translation activity.

Protein Expression in Different Cell Lines

Fluc mRNA demonstrates reliable expression performance across various mammalian cell lines, supporting broad applicability in cell-based assays.

In Vivo Bioluminescence Imaging of mRNA Expression Over Time

mRNA Bulk Solution Stability

Stability at 2–8°C

Freeze-thaw cycle stability

Long-term storage stability at -80°C

FAQ

Q1: What is Fluc mRNA used for?

Fluc mRNA is primarily used as a reporter gene tool for evaluating mRNA expression efficiency, delivery systems, gene regulation, and protein expression in cell and animal models.

Q2: How does Fluc mRNA generate detectable signals?

After translation, firefly luciferase catalyzes the oxidation of luciferin to produce bioluminescence, which can be quantitatively measured using a luminometer or imaging system.

Q3: What is the storage condition for Fluc mRNA?

Fluc mRNA should be transported at -45°C to 25°C and stored at -85°C to -65°C.

Q4: How stable is Fluc mRNA?

The product remains stable for 1 month at 2–8°C and tolerates more than 10 freeze-thaw cycles under validated conditions.

Q5: Can Fluc mRNA be customized?

Yes. Customization services are available, including sequence design and mRNA modification options.

Related Products