Product Overview

Cre mRNA (modified) encodes Cre recombinase, a site-specific DNA recombinase derived from bacteriophage P1. By specifically recognizing loxP sites, Cre recombinase enables targeted DNA recombination events, including deletion, inversion, and translocation of DNA fragments flanked by loxP sequences.

The Cre-loxP system is a powerful genetic engineering tool widely used for conditional gene regulation, inducible gene knockout, lineage tracing, and construction of genetically modified animal models.

This product is an N1-methylpseudouridine (N1-Me-pUTP) modified mRNA synthesized through in vitro co-transcriptional capping and purified using precipitation and desalting technologies. The modification reduces innate immune activation while maintaining high translation efficiency in mammalian cells.

With a theoretical length of 1343 nt, Cre mRNA (modified) provides enhanced cellular performance and is suitable for applications requiring efficient protein expression and reduced immunogenicity.

Amino Acid Sequence of Encoded Protein：

MGTPKKKRKVRTDLNSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKMLLSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGLPRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAFLGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVERWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQRYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLEDGD*

Key Features

Reduced Innate Immune Activation

N1-methylpseudouridine modification helps reduce recognition by innate immune sensors and improves mRNA compatibility for cellular applications.

Enhanced Translation Efficiency

Modified nucleosides improve mRNA stability and support sustained Cre recombinase protein expression in mammalian cells.

Validated Functional Expression

Demonstrates efficient Cre protein production after transfection, supporting reliable Cre-loxP recombination studies.

High-Quality Manufacturing and Supply

Produced with optimized mRNA synthesis and purification workflows, with multiple specifications and customization options available.

Applications

Cre mRNA (modified) is suitable for:

• Cre-loxP based conditional gene editing

• Transient Cre recombinase expression studies

• Gene knockout and gene activation research

• Genome engineering workflows

• Animal model development and validation

• Cell-based and in vivo recombination assays

• Advanced gene therapy and RNA delivery research

Performance Data

Detection of Protein Expression by Western Blot Analysis

Cre mRNA (modified) shows efficient Cre recombinase expression in mammalian cells following transfection.

Stability Evaluation

Stability of modified Cre mRNA bulk solution at 2–8°C

Freeze-thaw stability under repeated cycles

Long-term storage stability at -80°C

Comparative Performance

N1-Me-pUTP modified Cre mRNA provides improved translation performance and reduced immunogenicity compared with unmodified mRNA.

FAQ

Q1: What is Cre mRNA (modified)?

Cre mRNA (modified) is an N1-methylpseudouridine-modified mRNA encoding Cre recombinase for Cre-loxP mediated genome engineering applications.

Q2: Why use N1-methylpseudouridine modification?

N1-Me-pUTP modification can reduce innate immune recognition, improve mRNA stability, and enhance translation efficiency in mammalian cells.

Q3: What applications are suitable for modified Cre mRNA?

It is suitable for conditional gene regulation, genome editing research, animal model studies, and in vivo RNA delivery applications.

Q4: How should the product be stored?

The recommended transportation condition is -45°C to 25°C, and storage at -85°C to -65°C is recommended for long-term stability.

Q5: Is customization available?

Yes. Sequence optimization, formulation adjustment, modification type, and packaging customization are available.

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