Product Overview

Cre mRNA encodes Cre recombinase, a 38 kDa site-specific DNA recombinase derived from bacteriophage P1. Cre recombinase specifically recognizes loxP sites and catalyzes recombination between two loxP sequences, enabling targeted DNA excision, inversion, or translocation.

The Cre-loxP system is one of the most widely used genome engineering technologies and is extensively applied in conditional gene expression regulation, gene knockout studies, gene function analysis, and the generation of genetically modified animal models.

This product is an mRNA synthesized through in vitro co-transcriptional capping and purified by precipitation and desalting processes. The mRNA has a theoretical length of 1343 nt and supports efficient protein expression in mammalian cells, making it suitable for cell-based assays and in vivo research applications.

The encoded Cre recombinase contains nuclear localization signals (NLS), enabling efficient nuclear transport after translation and facilitating Cre-mediated recombination at target loxP sites.

Amino Acid Sequence of Encoded Protein：

MGTPKKKRKVRTDLNSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKMLLSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGLPRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAFLGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVERWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQRYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLEDGD*

Key Features

Efficient Cre Recombinase Expression

Optimized mRNA design enables robust translation of functional Cre recombinase protein in mammalian cells for downstream recombination studies.

High Purity and Reliable Performance

Produced through optimized in vitro transcription, capping, purification, and desalting processes to ensure high-quality mRNA suitable for research applications.

Broad Compatibility

Compatible with various cell transfection systems and animal models for conditional gene regulation and genome engineering studies.

Flexible Product Availability

Available in different packaging sizes with customization services and reliable supply support.

Applications

Cre mRNA is suitable for a wide range of molecular biology and genetic engineering applications, including:

• Cre-loxP mediated genome editing

• Conditional gene knockout and gene activation studies

• Temporal and spatial gene regulation research

• Generation and validation of genetically modified animal models

• Functional genomics studies

• Cell-based recombination assays

• In vivo gene manipulation studies

Performance Data

Detection of Protein Expression by Western Blot Analysis

Cre mRNA demonstrates efficient protein expression following transfection into mammalian cells.

mRNA Stability Analysis

Stability of Cre mRNA bulk solution at 2–8°C

Freeze-thaw stability analysis of Cre mRNA bulk solution

Long-term stability of Cre mRNA bulk solution at -80°C

FAQ

Q1: What is Cre mRNA used for?

Cre mRNA is mainly used to express Cre recombinase for Cre-loxP mediated DNA recombination, including conditional gene knockout, gene regulation, and genome engineering studies.

Q2: What is the advantage of using Cre mRNA instead of Cre protein or plasmid DNA?

Cre mRNA enables transient Cre recombinase expression without genomic integration risks associated with DNA-based delivery methods, making it suitable for controlled genome engineering applications.

Q3: What are the storage and transportation conditions?

The recommended transportation condition is -45°C to 25°C, and long-term storage is recommended at -85°C to -65°C.

Q4: Can Cre mRNA be customized?

Yes. Customized sequences, modifications, and packaging specifications are available upon request.

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