T7 RNA Polymerase (Trace dsRNA)

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100μL: HBP000360-1

1mL: HBP000360-2

10mL: HBP000360-3

100mL: HBP000360-4

Cat. No: HBP000360

Transportation and storage: Transportation under 0°C and storage at -25～ -15 °C.

Technical Support

Product Description

T7 RNA Polymerase is a DNA-dependent RNA polymerase from T7 phage, with highly specific 5 ´→ 3 ´ RNA polymerase activity, and fewer dsRNA byproducts in the in vitro transcriptional product compared to wild-type T7 RNA polymerase.

GMP-Grade T7 RNA Polymerase

During in vitro transcription, T7 RNA polymerase binds to the T7 promoter sequence in the DNA template to amplify a large amount of mRNA. Haymes Biotech provides wild-type and more than 30 engineered T7 RNA polymerase mutants to meet the needs of different application scenarios.

Key Features

• High Fidelity: no significant difference in fidelity compared to wild-type T7 RNA by Sanger sequencing.

• Strict QC panel: multi-dimensional quality control of enzymes, CV≤15%.

• 30+ standard mutants available: High mRNA Integrity Mutant: Cat No. HBP000330 /Low dsRNA Mutant13: Cat No. HBP000340/Thermostable Mutant: Cat No. HBP000350.

• GMP-Grade, Non-Animal Origin (AOF), DMF Record: MF036856.

Quality Control

Multi-dimensional quality control standard to ensure good consistency between batches and to secure the successful application in pharmaceutical industry.

Category	Parameter	Batch Number
Category	Parameter	H20220801B	HH20220802	HH20220803
Industry Standards	Appearance	Clear Colorless Solution	Clear Colorless Solution	Clear Colorless Solution
	Purity	≥95%	98. 4%	98. 7%
	Enzyme Activity	50U/μl	50. 5U/μl	51. 7U/μl
	Promoter Specificity	No non-specific transcript products	No non-specific transcript products	No non-specific transcript products
	RNase Residual	Non detected	Non detected	Non detected
	Nickase Residual	Non detected	Non detected	Non detected
	Endonuclease Residual	Non detected	Non detected	Non detected
	Exonucleases Residual	Non detected	Non detected	Non detected
	HCD Residual	≤0. 1pg/50U	0. 012/50U	0. 018/50U
	HCP Residual	≤50ppm	Non detected	Non detected
	Endotoxin	≤10EU/mg	≤10EU/mg	≤10EU/mg
	Microbial load	Non detected	Non detected	Non detected
Extra internal control Standard	HBV/HCV/HIV	Negative	Negative	Negative
	Mycoplasma	Negative	Negative	Negative
	Heavy Metal Residue	≤10ppm	≤10ppm	≤10ppm

Product Name	Catalog No.	Specification	Packing Specifications
T7 RNA Polymerase (low dsRNA)	HBP000340-1	500/μL	100μL
	HBP000340-2	500/μL	1mL
	HBP000340-3	500/μL	10mL
	HBP000340-4	500/μL	100mL
T7 RNA Polymerase (thermostable)	HBP000350-1	500/μL	100μL
	HBP000350-2	500/μL	1mL
	HBP000350-3	500/μL	10mL
	HBP000350-4	500/μL	100mL