Product Overview

L-γ-Glutamyl-3-carboxy-4-nitroanilide ammonium salt (Glupa-C) is a highly specific and stable substrate dedicated to γ-glutamyltransferase (GGT) assays (CAS: 67836-99-5). Manufactured via low-temperature condensation and special decolorization processes, it features ≥99% purity, ultra-low background noise, and excellent batch-to-batch consistency. Compatible with fully automated clinical chemistry analyzers, it is widely used for quantitative determination of GGT activity in serum/plasma, supporting liver and biliary disease diagnosis, enzymatic research, and large-scale diagnostic reagent production.

Key Features

High Purity & Ultra-Low Blank

≥99% purity. Proprietary decolorization eliminates key impurities including pNP to cut blank absorbance and avoid false-positive results.

Excellent Batch-to-Batch Consistency

Customized synthesis processes ensure minimal inter-batch variation, guaranteeing stability during long-term reagent storage and large-scale production.

Fully Compatible with Automated Assays

Maximum absorbance at 405 nm, perfectly matching the detection channels of mainstream fully automated clinical chemistry analyzers for direct use in routine GGT assays.

Stable & Controllable Supply

Key raw materials are self-produced, with an independently controllable supply chain to avoid disruption risks and ensure long-term stable delivery.

Dual Qualified Inspection

Besides routine physicochemical tests, additional biochemical applicability evaluation is conducted to meet dual quality specifications.

Applications

Clinical Diagnostic Reagents:

Core substrate for GGT activity assays in serum/plasma, compatible with various fully automated clinical chemistry analyzers, supporting the screening and diagnosis of liver and biliary diseases, alcoholic liver disease, biliary obstruction, and other conditions.

Enzymatic Research:

Standard substrate for γ-glutamyltransferase activity determination, used in enzyme kinetic analysis, inhibitor screening, and related metabolic pathway studies.

Diagnostic Reagent Manufacturing:

Provides high-quality, batch-stable raw materials for in vitro diagnostic reagent manufacturers, supporting large-scale production and quality control of GGT assay kits.

Specification

Item	Specification
Product Name	L-γ-Glutamyl-3-carboxy-4-nitroanilide ammonium salt (Glupa-C)
English Synonyms & Search Keywords	GGT substrate, Gamma-glutamyl transferase substrate, γ-GT substrate, GGT diagnostic substrate, Glu-CPNA ammonium salt
Catalog No.
CAS No.	67836-99-5
Molecular Formula	C12H16N4O7
Molecular Weight	328.28
Purity	≥99% (HPLC)
Appearance	White to light yellow powder
λmax	405 nm (Colorimetric wavelength)
Storage	Short-term storage: 2-8°C, sealed, dry, and protected from light. For long-term storage, it is recommended to store at -25 to -15°C, also protected from light.
Product Line	Clinical chemistry enzyme substrates

Performance Data

Purity Comparison

A Brand

Hzymes

Verified by HPLC testing, our product features high purity and minimal impurities. The critical impurity 5-amino-2-nitrobenzoic acid is maintained at an ultra-low level, ensuring superior overall quality versus competing products.