Product Overview
Universal Fast One-Step RT-qPCR Probe Mix U+ (One Tube) is a probe-based multiplex real-time RT-qPCR master mix developed for rapid and sensitive RNA detection. The optimized formulation integrates reverse transcription and quantitative PCR into a single-tube workflow, reducing hands-on time and minimizing contamination risks.
The mix contains UDG and dUTP carryover prevention technology, enabling effective degradation of contaminating amplification products from previous reactions at room temperature. It also supports pre-mixing of buffer, enzymes, primers, and probes, making it ideal for molecular diagnostic assay development and high-throughput testing workflows.
Designed for fast cycling protocols, this one-step RT-qPCR reagent delivers reliable performance across a wide range of RNA detection applications, including respiratory viruses, enteric pathogens, hepatitis viruses, and other RNA targets.
Benefits
• One-step RT-qPCR workflow for RNA detection
• Supports multiplex fluorescent probe assays
• UDG/dUTP system minimizes carryover contamination
• Compatible with rapid PCR protocols
• Supports primer-probe premix preparation
• High sensitivity for low-copy RNA targets
• Stable formulation for assay development and routine testing
Key Features
High Sensitivity for Low-Copy RNA Detection
The optimized enzyme and buffer system enables reliable detection of single-digit copy RNA templates, helping improve positive sample detection rates and assay confidence.
Ultra-Fast RT-qPCR Workflow
The master mix supports rapid amplification protocols, allowing results to be obtained in as little as 30 minutes, helping laboratories increase throughput and shorten turnaround times.
UDG/dUTP Carryover Contamination Prevention
Built-in Uracil-DNA Glycosylase (UDG) and dUTP effectively eliminate carryover contamination from previous PCR amplifications, improving assay reliability and reducing false-positive risks.
Broad Assay Compatibility
The reagent is designed to support a wide range of RNA detection projects and multiplex probe-based assays.
Premix-Friendly and Easy to Use
The formulation allows pre-mixing of primers and probes together with the reaction components, simplifying assay preparation and improving workflow efficiency.
Excellent Stability
The reagent maintains performance after storage at 37°C for up to 10 days, supporting transportation, assay development, and routine laboratory use.
Applications
Universal Fast One-Step RT-qPCR Probe Mix U+ (One Tube) is designed for RNA detection assays based on quantitative real-time PCR (RT-qPCR) technology and is suitable for assay development, validation, and routine testing workflows.
• Pathogen nucleic acid detection based on real-time RT-qPCR methodology
• Probe-based multiplex RT-qPCR assays
• RNA target detection and quantification
• Molecular diagnostic assay development
• High-throughput RT-qPCR testing workflows
• Research applications involving RNA analysis
Customer Case
Case 1: SARS-CoV-2 Nucleic Acid Testing
Targets: FAM-ORF1ab, ROX-N, HEX-Human Internal Reference
Instrument: SLAN-48S
Program Total Duration: 27 min
Test Protocol: Prepare fully premixed reaction solution containing buffer, enzyme mix, primers and probes with HMD6902T, followed by incubation at 37°C for 10 days. The control group adopts freshly prepared reaction system. SARS-CoV-2 nucleic acid reference materials at concentrations of 1E4 copies/mL and 5E2 copies/mL are used as templates.
Case 2: Influenza A and B Virus Nucleic Acid Detection
Targets: FAM-Influenza A, HEX-Influenza B, CY5-Human Internal Reference
Instrument: SLAN-48S
Program Total Duration: 27 min
Test Protocol: Prepare fully premixed reaction solution containing buffer, enzyme mix, primers and probes with HMD6902T, followed by incubation at 37°C for 10 days. The control group adopts freshly prepared reaction system. SARS-CoV-2 nucleic acid reference materials at concentrations of 1E4 copies/mL and 5E2 copies/mL are used as templates.
