Hot Start Taq DNA Polymerase ( Antibody modification ) is a heat-resistant DNA polymerase derived from Thermus aquaticus YT-1. The enzyme has 5'→3' polymerase activity and double-strand specificity 5 '→ 3'exonuclease activity. The enzyme uses antibody modification to block low temperature and room temperature enzyme activity. After thermal activation at 95°C, the antibody falls off to release the enzyme activity. Before the amplification system is formulated to PCR amplification, non-specific amplification due to primer mismatch will not occur, which minimizes the formation of non-specific amplification and primer dimers, and improves the sensitivity and specificity of amplification. The product uses a specially optimized reaction buffer, and HzymesAccu AM Hot Start Taq DNA Polymerase to make a pre-mixed reagent, which maximizes the stability of the enzyme during storage. Only the primer template and water are added before configuring the system, which greatly simplifies The preparation steps.
Product Name | Cat. No | Size | No. of Reactions |
Hot Start PCR Master Mix (Antibody modified) | HMD3801-01 | 1ml | 100rxn |
HMD3801-02 | 5×1ml | 500rxn | |
HMD3801-03 | 10ml | 1000rxn | |
HMD3801-04 | 50ml | 5000rxn |
Transportation and storage methods
≤0℃ for transportation; -25~-15℃ for storage.