Product Overview

Recombinant Murine RNase Inhibitor is a high-purity functional protein produced by recombinant expression in E. coli and optimized for RNA protection in molecular biology, molecular diagnostics, and mRNA development workflows.

This RNase inhibitor protects RNA integrity by specifically binding to RNase A, RNase B, and RNase C through a 1:1 stoichiometric non-covalent interaction, effectively blocking ribonuclease activity and preventing RNA degradation. It does not inhibit RNase 1, RNase T1, S1 Nuclease, RNase H, or Aspergillus-derived RNase, ensuring reliable performance in diverse RNA-related applications.

Compared with conventional human RNase inhibitors, recombinant murine RNase inhibitor eliminates two oxidation-sensitive cysteine residues responsible for inhibitor inactivation. This structural optimization provides enhanced stability under low DTT conditions (<1 mM), making it particularly suitable for sensitive applications such as real-time RT-PCR, reverse transcription, and in vitro transcription (IVT) workflows where reducing agents need to be minimized.

With excellent enzyme activity, high purity, and GMP-oriented quality control, Hzymes Recombinant Murine RNase Inhibitor provides a reliable solution for RNA protection in research and biopharmaceutical development.

Key Features

GMP-Grade Quality and Reliable Manufacturing

• Produced with an animal-origin-free manufacturing process

• Free of β-lactam antibiotics

• Manufactured under strict quality control standards to ensure high batch-to-batch consistency

• Supported by DMF filing for GMP applications

High Purity with Low Residual Impurities

• Nuclease-free formulation to protect RNA integrity

• Extremely low levels of residual impurities, including:

• Host cell DNA (HCD)

• Host cell proteins (HCP)

• Microorganisms

• Metal ions

• Endotoxin

Superior Stability Under Low DTT Conditions

• Optimized murine-derived structure eliminates oxidation-sensitive cysteine residues

• Maintains excellent activity under low reducing agent conditions (<1 mM DTT)

• Suitable for RT-PCR and other oxidation-sensitive RNA workflows

High Activity and Exceptional Purity

• Protein purity >99%

• Higher enzymatic activity compared with imported benchmark products

• Delivers consistent performance across different production batches

Broad Compatibility and Excellent Functional Performance

• Compatible with mainstream reverse transcriptase, DNA polymerases, and RNA polymerases

• Provides superior RNA protection performance at equivalent enzyme activity levels

• Suitable for demanding molecular biology and biopharmaceutical applications

Applications

RT-PCR and RT-qPCR

Recombinant Murine RNase Inhibitor effectively protects RNA templates during reverse transcription and amplification processes, improving RNA stability and ensuring reliable RT-PCR and RT-qPCR results.

In Vitro Transcription (IVT) and mRNA Development

This RNase inhibitor is designed for mRNA synthesis workflows, helping maintain RNA integrity during IVT reactions and supporting the development of mRNA vaccines and therapeutic RNA products.

cDNA Synthesis and RNA Library Construction

Provides efficient RNase protection during cDNA preparation, RNA library construction, and other RNA molecular biology workflows.

mRNA Vaccine and Gene Therapy Manufacturing

With GMP-grade quality control and excellent stability, this product is suitable for preclinical research, process development, and large-scale manufacturing workflows for mRNA vaccines and gene therapy products.

Performance Data

Efficient RNase A Inhibition and RNA Protection

Hzymes Recombinant Murine RNase Inhibitor effectively inhibits RNase A activity and protects RNA integrity under RNase challenge conditions, ensuring reliable RNA preservation.

Superior Stability Compared with Competitors

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Hzymes Recombinant Murine RNase Inhibitor maintains excellent RNA protection performance after freeze storage (-20°C), heat treatment (55°C, 5 min), and repeated freeze-thaw cycles, outperforming commercial competitors.

High Functional Activity

Provides strong RNase inhibition capacity with efficient protection of RNA substrates, supporting reliable performance in RT-PCR, RT-qPCR, and IVT mRNA workflows.

FAQ

Q1: What is Recombinant Murine RNase Inhibitor used for?

Recombinant Murine RNase Inhibitor is used to protect RNA from degradation by inhibiting ribonuclease activity. It is widely applied in RT-PCR, RT-qPCR, cDNA synthesis, IVT mRNA synthesis, and RNA library construction workflows.

Q2: How does Murine RNase Inhibitor protect RNA?

The inhibitor binds RNase A, RNase B, and RNase C through a 1:1 non-covalent interaction, blocking their ribonuclease activity and preventing RNA degradation.

Q3: What RNases are inhibited by this product?

This product effectively inhibits RNase A, RNase B, and RNase C, while showing no inhibitory effect on RNase 1, RNase T1, S1 Nuclease, RNase H, and Aspergillus-derived RNase.

Q4: What are the advantages of Murine RNase Inhibitor compared with human RNase inhibitors?

The murine variant removes two oxidation-sensitive cysteine residues found in human RNase inhibitors, providing improved stability, especially under low DTT conditions (<1 mM).

Q5: What are the storage and transportation conditions?

Transportation is recommended under frozen conditions below 0°C. Store the product at -25°C to -15°C.

Q6: Is this product suitable for GMP applications?

Yes. The product is manufactured with GMP-oriented quality control, is animal-origin-free, free of β-lactam antibiotics, and supported by DMF filing, making it suitable for GMP manufacturing and biopharmaceutical development.

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