Production Overview

Hzymes’ T7 RNA Polymerase ELISA kit uses a double antibody sandwich enzyme-linked immunosorbent assay to detect the residual amount of T7 RNA polymerase.

Key Features

• Easy to use with pre-coated plates.

• One-step method, requiring only one sample addition and one plate wash to develop color.

• Short detection time, with a total reaction time of 1h 15min.

Product Highlights

• High sensitivity: LOD is ＜0.1 ng/mL，LOQ=0.25 ng/mL

• High accuracy: recovery between 80%-120%

• High precision: intra-assay CV≤10%, inter-assay CV ≤15%, ensuring the repeatable data

• High specificity: only specific recognition of T7 RNA polymerase, other IVT tool enzymes do not interfere with the measurements.

• Good compatibility: Hzymes’ kit is compatible with most commercially available T7 RNA polymerases.

• Excellent anti-interference performance: actual measurements of 1–2 mg/mL mRNA samples showed acceptable recovery rates (80–120%) after dilution at 2×, 4×, 10×, 25×, 50×, 100×, 200×, 400×, and 800×.

Applications

Assay to accurately measures specific residual proteins of T7 RNA polymerase, providing detailed insights into process impurities.

Performance Data

Limit of Detection (LOD)

As low as 0.1ng/mL and Limit of Quantification (LOQ) as low as 0.25 ng/mL.

High accuracy and repeatability

CV＜10% with spike recovery in the range of 80-120%.

High specificity:

No cross-reactivity to other proteins.

Good adaption

Current T7 RNA Polymerase ELISA kit from Supplier A, B, C doesn’t match with multiple T7 RNA polymerase on the market.

Hzymes’ T7 RNA Polymerase ELISA kit is adapt to different T7 RNA polymerase from multiple suppliers.

Better anti-interference ability

Under the same dilution factor, Hzymes’ kit shows better recovery performance.

Good data and batch repeatability.

Mouse Epo Mrna sample at 1mg/mL concentration for 3 times.

Three samples were tested using kits of three batches.

Customer Case

We have cumulatively facilitated multiple IND applications in both China and the United States, covering cutting-edge fields such as mRNA vaccines, therapeutic mRNA for oncology, and in vivo CAR-T cell therapy.

FAQ

Is this kit GMP grade or for research use only?

This kit is for research use only, but has successfully been used for several IND application cases in both China and United States.

Can this kit be adapted to the T7 RNA polymerase we use?

Hzymes’ T7 RNA polymerase kit has been verified that it can be adapt to most T7 RNA polymerase on the market, but we still recommend you to do the recovery test to check whether you can use this kit directly or you need to use your T7 RNA polymerase to set the standard curve.