Product Overview

Endo H is a recombinant glycosidase cloned from Streptomyces plicatus and overexpressed in E. coli. It cleaves the chitobiose core of high-mannose oligosaccharides and a limited number of hybrid oligosaccharides from asparagine-linked glycoproteins, it does not cleave complex-type glycan chains.

One unit of enzyme activity is defined as the amount of enzyme required to remove >95% of the carbohydrates from 10 μg of denatured RNase B in a total reaction volume of 10 μl within 1 hour at 37°C.

Key Features

Recombinant Expression in E. coli

Produced in E. coli as a recombinant glycosidase derived from Streptomyces plicatus, with no detectable contamination from exoglycosidases or other glycosidase activities.

Tag-Free Protein Design

Expressed without purification tags, facilitating downstream separation in workflows involving affinity-tagged proteins.

High Purity

Purity is typically >95% as assessed by SDS-PAGE analysis.

Good Activity and Storage Stability

Demonstrates consistent enzymatic activity under recommended conditions and is stable for up to 2 years when properly stored.

Applications

Antibody and Glycoprotein Glycan Analysis

Used for profiling high-mannose and certain hybrid N-linked glycans on antibodies and glycoproteins, supporting structural and functional studies.

Enzymatic Glycoengineering Workflows

Can be used in combination with enzymes such as Endo S and GalT in antibody glycan remodeling and conjugation strategies based on N-glycan structures.

Performance Data

FAQ

What are the recommended storage conditions and shelf life of this product?

Store at -20°C. For long-term storage, keep the product tightly sealed and avoid repeated freeze-thaw cycles.

What is the recommended usage or activity definition for this product?

One unit of Endo H activity is defined as the amount of enzyme required to remove more than 95% of glycans from 10 μg of denatured RNase B in a total reaction volume of 10 μL within 1 hour at 37°C.

Do you provide OEM or private-label manufacturing services?

Yes, OEM and private-label services are available upon request.

What are the shipping conditions and typical lead times?

For in-stock products, the typical lead time is 2-3 weeks. For out-of-stock items, lead time is approximately 8-12 weeks depending on production scheduling and order volume.